碳酸盐与磷酸盐对Biohydrogenbacterium R3 sp.nov.产氢发酵效能的影响

Influence of carbonates and phosphates on fermentative biohydrogen production of Biohydrogenbacterium R3 sp.nov.

采用NH4HCO3、NaHCO3、Na2CO3、K2HPO4、KH2HPO4、NaH2PO4和Na2HPO4作为碳酸盐和磷酸盐缓冲溶液源,研究其对Biohydrogenbacterium R3 sp.nov.产氢发酵效能的影响.研究发现,碳酸盐对Biohydrogenbacterium R3 sp.nov.反应体系中的pH值都具有较好的缓冲作用,而磷酸盐的添加对其产氢效能的促进作用较明显.反应器内添加了Na2HPO4与K2HPO4的Biohydrogenbacterium R3 sp.nov.的氢气产量与氢气含量达到最高值,分别为1978.56 mL、44.1%与2160.9 mL、45.8%,此时反应器内的pH值分别为3.28和3.41,细胞浓度分别为1.03 g·L-1和1.21 g·L-1,添加了Na2HPO4的Biohydrogenbacterium R3 sp.nov.的乙醇和乙酸产量分别为4841.49 mg·L-1和2064.94 mg·L-1;而添加了Na2CO3的氢气产量、氢气含量、细胞浓度与反应器内pH值分别为1064.42 mL、35.96%、1.23 g·L-1与3.81,此时乙醇、乙酸的产量分别为3862.21 mg·L-1和1930.86mg·L-1.

This study investigated the effects of phosphates and carbonates （ NH4 HCO3 , NaHCO3 , Na2CO3 , K2HP04, KI-I2PO4, NaH2PO4 and Na2 HPO4 ） on the hydrogen production efficiency and cell growth to Biohydrogenbacterium R3 sp. nov. It was indicated that the carbonates could buffer the pH in reactor better and the phosphates could promote the hydrogen production obviously. When Na2 HPO4 and K2 HPO4 were added into the reactor, the hydrogen production and content reached the maximum of 1978.56 mL, 44. 1% and 2160.9 mL, 45.8% , respectively. The pH and cell weight achieved 3.28,1.03 g·L^-1 and 3.41, 1.21 g·L^-1, respectively. When Na2 H HPO4 was added into the reactor, the ethanol and acetic acid were 4841.49 mg·L^-1 and 2064.94 mg·L^-1, respectively, while when Na2 CO3 was added into the reactor, the hydrogen production, hydrogen content, cell weight, pH, ethanol and acetic acid were 1064.42 mL, 35.96% , 1.23 g·L^-1 , 3.81, 3862.21 mg·L^-1 and 1930.86 mg·L^-1 , respectively.