惊厥性疾病患儿血浆和脑脊液中神经肽Y、脑源性神经营养因子、白介素-6的水平变化及相关性研究

Neuropeptide Y,Brain-derived neurotrophic factor,Interleukin-6 in plasma and cerebrospinal fluid of children with convulsive diseases

目的:探讨惊厥性疾病患儿血浆和脑脊液中神经肽Y(NPY)、脑源性神经营养因子(BD-NF)、白介素-6(IL-6)的水平变化及相互关系。方法:选取惊厥性疾病患儿74例,分别在入院第1天、第7天采取静脉血,第7天采取脑脊液(CSF)标本,采用放射免疫分析方法检测血浆和CSF中NPY、BDNF、IL-6的水平。结果:惊厥组(热惊厥、癫痫)患儿惊厥发作后血浆、CSF中NPY、Il-6水平均增高,BDNF降低,但三者第7天较第1天增高;血浆、CSF中NPY、BDNF在不同热惊厥发作和癫痫发作中无差异,但IL-6在不同热惊厥发作和癫痫发作中有差异;血浆、CSF中NPY、IL-6与惊厥中发作次数有关,BDNF与惊厥中发作次数无关;血浆与CSF中的NPY、BDNF、IL-6均分别呈正相关,血浆和CSF中的NPY与IL-6呈正相关,与BDNF呈负相关;BDNF与IL-6呈负相关。结论惊厥性疾病患儿血浆、CSF中NPY、Il-6水平均增高,与惊厥发作次数有关,在惊厥发生的不同阶段水平不同;血浆与CSF中NPY、BDNF、IL-6水平均分别呈正相关,血浆中的水平能间接反映CSF水平;血浆和CSF中的NPY与IL-6呈正相关,与BDNF呈负相关;BDNF与IL-6呈负相关。

Objective ：In order to study the levels and relationship of neuropeptide Y（NPY）, brainderived neurotrophic factor（BDNF）, interleukin 6 （IL-6）in plasma and cerebrospinal fluid（CSF） in children convulsion. Methods： 74 children with convulsive diseases were studied as following： obtaining the samples of plasma in the first day and the seventh day after being in hospital, and the samples of CSF in the 1st after being in hospital. We investigated the changes of NPY, BDNF, IL-6 in plasma and CSF with radioimmunoassay（RIA）. Results.. In children with convulsive diseases （ febrile seizure, epilepsy ） NPY, I1-6 in plasma and CSF were increased, but BDNF decreased. NPY, IL-6 and BDNF were higher in the seventh day than those in the first day in plasma and CSF. NPY and BDNF in different types of febrile seizures and epi leptic seizures were no difference, but IL-6 had differences. The levels of NPY, IL-6 in plasma and CSF correlated with the times of convulsion,but BDNF had no correlation to the times of convulsion. NPY, BDNF and IL-6 in plasma positively correlated with those in CSF respectively; In plasma and CSF NPY had significantly positive correlation to IL-6, and nega- tive correlation to BDNF; BDNF was negatively correlation to IL-6. Conclusion..NPY, IL-6 in plasma and CSF are elevated in children with convulsive diseases and have correlation to the times of convulsion, changing in the different course of convulsion. There are positive correlation in NPY, BDNF, IL-6 between plasma and CSF. The levels of those in plasma could indirectly reflect the levels of those in CSF. In plasma and CSF NPY have significantly positive correlation to IL-6 and negative correlation to BDNF; BDNF is negatively correlation to IL-6