雌性激素对小鼠下丘脑腹内侧核神经元多聚核糖体的影响

The effect of estrogen on polyribosomes in the ventromedial hypothalamic nucleus neurons in female mice

目的 :电镜观测苯甲酸雌二醇 ( EB)和已酸孕酮 ( HPC)对雌性小鼠下丘脑腹内侧核神经元多聚核糖体的影响。方法 :根据雌鼠的动情周期 ,对切除卵巢的幼鼠 ( OVX)每 5d分别肌肉注射 1次 8μg EB,2 mg HPC,8μg EB加 2 mg HPC。疗程 2个月。结果 :( 1 )神经元胞质中游离型多聚核糖体每 μm2的含量在 OVX组与正常组 ( INT)、OVX+ EB组 ,OVX+ HPC组和 OVX+ EB+ HPC组比较均无显著差异 ( P>0 .0 5)。 ( 2 )附着粗面内质网膜表面的结合型多聚核糖体每μm含量在OVX组分别比 INT组 ,OVX+ EB组 ,OVX+ HPC组和 OVX+ EB+ HPC组少 2 9.8%、2 6.2 %、35.2 %和 33.7%均有非常显著差异 ( P<0 .0 0 5)。结论 :本实验结果证实雌性激素特别是孕酮在小鼠生育早期使下丘脑腹内侧核神经元结合型多聚核糖体含量增多。

Objective:The effects of estradioli benzoatis (EB) and hydroxyprogesteroni caproatis (HPC) on polyribosomes in the ventromedial hypothalamic nucleus neurons in female Kunming mice were investigated with electron microscope Methods:Ovariectomized young mice (OVX) were treated (i m ) with (1) 8 μg EB(2) 2 mg HPC(3) 8μg EB and 2mg HPC,respectively every other five days,in a course of two months Results:(1)There was no significant differences among free polyribosmes content/μm 2 in the cytoplasm of OVX group,intact (INT) group, OVX+EB group ,OVX+HPC group and OVX+EB+HPC group ( P >0 05)(2) The bound polyribosomes content/μm,presented on the surface of the rough endoplasmic reticulum of OVX group was reduced by 29 8%,26 2%,35 2% and 33 7% respectively as compared with that of INT group,OVX+EB group ,OVX+HPC group and OVX+EB+HPC group ( P <0 005) Conclusion These results corroborated that estrogen,especially progesterone,increased bound polyribosomes contents of the ventromedial hypothalamic neurons during early breeding period of female Kunming mice