应用ISSR分析亚麻种质资源遗传多样性

Genetic Diversity of Flax Germplasm Resources Based on ISSR Markers

从供试材料中筛选到具有多态性的ISSR引物10条,利用这些引物对来自7个国家的48个亚麻品种的遗传多样性进行ISSR分析,共扩增到82条清晰的多态性条带,多态性比率为91.1%。用UPGMA法将48个亚麻品种聚为六大类,聚类结果表明地理位置相近的品种基本聚为一类,本研究结果可以指导亚麻育种亲本选配。

A total of 10 ISSR primers with polymorphism were identified. The genetic diversity was analysed based on the 10 primers for 48 flax varieties which were introduced from 7 countries. 82 polymorphic bands were amplified, and the percentage of polymorphic bands （PPB） was 91.1%. According to the clustering analysis with UPGMA method, 48 flax varieties were clustered into 6 groups and the varieties originated from the same geographical location were almostly clustered into one group. These resuits would provide instruction for selecting ideal parents to breed good flax variety.