外周血CD4^+CD25^(high)调节性T细胞表达及TGF-β1水平在壹期煤工尘肺中的变化

Changes of peripheral blood CD4^+CD25^(high) regulatory T cells and serum TGF-β1 levels in stage Ⅰ coal worker's pneumoconiosis

目的探讨外周血CD4+CD25high调节性T细胞(CD4+CD25highTreg)表达及转化生长因子β1(TGF-β1)水平在壹期煤工尘肺(CWP)中的变化及临床意义。方法采用流式细胞术和双抗体夹心ELISA法检测42例单纯壹期CWP患者(CWP组)、39例壹期CWP并发症患者(CWP并发症组)、41例具有与CWP组相同接尘条件的井下接尘矿工(接尘对照组)及38例井上健康查体人员(正常对照组)外周血CD4+CD25highTreg及血清TGF-β1水平。结果与正常对照和接尘对照组比较,CWP及其并发症组CD4+CD25highTreg水平均升高,差异均具有统计学意义(P<0.01);CWP组TGF-β1水平高于正常对照组和CWP并发症组,差异也具有统计学意义(P<0.05);CWP组吸烟者CD4+CD25highTreg水平高于非吸烟者,差异有统计学意义(P<0.01);其他各组间比较,CD4+CD25highTreg及TGF-β1水平均无显著性变化(P>0.05)。CWP组CD4+CD25highTreg与TGF-β1水平不相关(r=0.165,P=0.527)。结论壹期CWP患者外周血CD4+CD25high调节性T细胞表达和血清TGF-β1水平升高,可能参与CWP的发生发展过程;血清TGF-β1水平可能与疾病严重程度有关,吸烟可影响CD4+CD25highTreg表达。

Abstract： Objective To explore the changes of peripheral blood CD4 ＋ CD25h^sh regulatory T cells （ CD4 ＋ CD25highTreg） and serum transforming growth factorβ1 （TGF-β1） levels in the patients with stage I coal worker＇s pneumoconiosis （CWP） and its significance. Methods The levels of peripheral blood CD4＋ CD25highTreg and serum TGF-β1 were determined in 42 stage I CWP patients （CWP group）, 39 stage I CWP with complications patients （complication group）, 41 miners （dust exposed control group） and 38 healthy controls by flow cytometry and sandwich ELISA. Results The results showed that com- pared with healthy control and dust exposed control groups, the levels of CD4＋ CD25high Treg were＂ significantly higher in CWP and CWP with complications groups （P 〈0. 01 ）, the level of TGF-β1 in CWP was also higher than that of healthy control and CWP with complications groups （P 〈 0. 05 ）. In CWP group, the CD4 ＋ CD25hiShTreg level in smoking patiens was significantly higher than that of non-smoking patients （P 〈0. 01 ）, but among other groups both in CIM＋ CD25high Treg and TGF-[31 levels there was no significant difference （P 〉 0. 05 ） and obvious correlation （r = 0. 165, P = 0. 527）. Conclusion The results suggested that the levels of peripheral blood CD4 ＋ CD25hiShTreg and serum TGF-131 were increased in stage I CWP patients, which may involve in the developing process of CWP; besides, serum TGF-I31 levels in CWP may be related to the severity de- gree of CWP and smoking may influence the expression of CD4＋ CD25high Treg.