供肺保存和再灌注期间的肺表面活性物质变化

The alteration of pulmonary surfactant after lung preservation and reperfusion

目的 探讨供肺保存和再灌注期间的肺表面活性物质变化。方法 实验动物分为对照组 (非缺血保存组 )和实验组 (缺血保存组 ) ,对保存和再灌注期间的肺组织和支气管肺泡灌洗液中的肺表面活性物质磷脂进行了分析。结果 与对照组相比 ,实验组保存后肺组织总磷脂 (TPL)和卵磷脂 (PC)含量明显降低 ,卵磷脂占总磷脂的比例 (PC/TPL)降低 ,卵磷脂与鞘磷脂比 (L/S)降低。实验组再灌注后肺组织和支气管肺泡灌洗液中的TPL、PC、PC/TPL、L/S均明显低于对照组。结论 肺表面活性物质 (SAM)改变是肺缺血和再灌注损伤的敏感指标 ,热缺血、冷保存和再灌注对SAM磷脂的含量、构成及功能都有损害。

Objective To study the alteration of pulmonary sufactant during lung preservation and reperfusion. Methods The animals were divided into control group (no ischemia preservation) and experimental group (ischemia preservation). The pulmonary surfactant phospholipid in lung tissue and bronchoalveolar lavage fluid were analyzed after preservation and reperfusion. Results The content of total phospholipids (TPL) and phosphatidylcholine (PC) in lung tissue, the ratio of PC to TPL (PC/TPL), the ratio of lecithin to sphing omyelin(L/S) decreased after lung preservation. The TPL, PC, PC/TPL, L/S of lung tissue and bronchoalveolar lavage fluid after reperfusion in experimental group were significantly lower than that in control group. Conclusions The pulmonary surfactant is a sensitive indicator of lung ischemia reperfusion injury. All periods of warm ischemia, cold preservation and reperfusion are harmful to the content and function of surfactant phospholipid.