凉血化瘀法干预老年性黄斑变性CNV与外周血CCR3表达的相关研究

Study of liangxue huayu in treatment of CNV in exudative age-related macular degeneration and expression of CCR3 on peripheral blood mononuclear cells

目的:探讨凉血化瘀法治疗渗出型老年性黄斑变性CNV与外周血T淋巴细胞及嗜酸性粒细胞表面CCR3表达的关系。方法:以32例38眼渗出型老年性黄斑变性患者、44例73眼萎缩型老年性黄斑变性患者及50例健康人为观察对象,流式细胞术检测外周血T淋巴细胞及嗜酸性粒细胞表面CCR3的表达,对渗出型老年性黄斑变性患者予凉血化瘀中药治疗,比较治疗前后患者外周血CCR3的表达。结果:渗出型老年性黄斑变性患者外周血T淋巴细胞及嗜酸性粒细胞表面CCR3表达均高于健康对照组(P<0.01)。萎缩型老年性黄斑变性患者外周血CCR3表达和健康对照组没有显著性差异(P>0.05)。渗出型老年性黄斑变性患者治疗前后外周血CCR3的表达没有显著性差异(P>0.05)。结论:渗出型老年性黄斑变性CNV的形成可能与外周血T淋巴细胞及/或嗜酸性粒细胞表面CCR3的高表达有关,而凉血化瘀法能有效治疗渗出型老年性黄斑变性CNV,却与CCR3表达不相关。

· AIM： To compare the expressions of CCR3 on T lymphocytes and/or eosinophil peripheral blood cell surface before and after liangxue huayu in treatment of choroidal neovascularization （CNV） in exudative age- related macular degeneration （AMD） and explore the mechanism of the incidence of CNV on exudative AMD. · METHODS： Totally 32 cases of exudative AMD patients, 44 cases of atrophic AMD patients and 50 healthy subjects were observed. 32 cases of exudative AMD patients were treated by liangxue huayu decoction and the expressions of CCR3 on T lymphocytes and/or eosinophil peripheral blood cell surface were detected by flow cytometry. · RESULTS： The expressions of CCR3 on T lymphocytes and/or eosinophil peripheral blood cell surface of the patients with exudative AMD were higher than the control group of healthy people （ P〈 0, 01 ） ; The expressions of CCR3 on T lymphocytes and/or eosinophil peripheral blood cell surface were no significant difference between the patients with atrophic AMD and the control group of healthy people （P〉0.05） · The expressions of CCR3 on T lymphocytes and/or eosinophil peripheral blood cell surface were no significant difference after liangxue huayu on treatment of CNV on exudative AMD （P〉0. 05）. · CONCLUSION： The CNV formation of exudative AMD may be related to the higher expressions of CCR3 on T lymphocytes and/or eosinophil cell surface. Liangxue huayu decoction is effective in treating exudative age-related macular degeneration CNV, but it is not related with the expression of CCR3.