血浆miR-328在心房颤动中的表达水平及其临床意义

The expression and clinical implication of plasma miR.328 in patients with atrial fibrillation

目的观察血浆miR-328在心房颤动（房颤）中的表达水平及其临床意义。方法选择58例房颤患者为房颤组（17例阵发性房颤、21例持续性房颤、20例永久性房颤），15例健康志愿者为对照组，收集两组一般临床资料及相关生化指标；运用实时荧光定量聚合酶链式反应法检测两组血浆miR-328的表达水平；同时结合相关指标，分析血浆miR-328与其相关性，评估血浆miR-328在房颤中的临床意义。结果（1）与对照组相比，房颤组血浆miR-328的表达水平显著升高，是对照组的（7．72±9．32）倍，差异有统计学意义（P〈0．05）。（2）房颤组中，阵发性房颤、持续性房颤及永久性房颤之间miR-328的表达水平（分别为：1．98±0．81、6．57±5．82和13．47±12．29）差异有统计学意义（P〈0．05），且随房颤持续时间的延长表达增加。（3）房颤组中，miR-328与左心房径呈正相关关系，相关系数为0．310（P〈0．05）。结论房颤患者血浆miR-328表达显著升高，可能参与了心房解剖重构。

Objective To observe the expression and clinical implication of plasma miR-328 in patients with atrial fibrillation （AF）. Methods Fifty-eight patients with AF （ AF group： 17 paroxysmal AF, 21 persistent AF, and 20 permanent AF） and 15 healthy volunteers （Control group） were included. General clinical data and related biochemical parameters were collected. Plasma miR-328 levels were detected with quantitative real-time polymerase chain reaction （qRT-PCR） analysis. The correlation between plasma miR- 328 and AF risk factors was analyzed. Results （ 1 ） Compared with the control group,the expression level of plasma miR-328 was significantly elevated in AF group （ fold 7. 72 ± 9. 32 ） （ P 〈 O. 05 ）. （2） In AF group, the expression of plasma miR-328 was significantly different in different type of AF [ paroxysmal AF with （ 1.98 ± 0. 81 ）, persistent AF with （6. 57 ± 5.82 ） and permanent AF with （13.47 ± 12. 29 ） ] （P 〈 O. 05 ）, and which was increased in proportion to the duration of AF. （ 3 ） There was a positive correlation between plasma miR-328 level and left atrial diameter in the AF group （ r = 0. 310, P 〈 0.05 ）. Conclusion miR- 328 expression is significantly increased in patients with AF, which may be involved in the atrial remodeling process of AF.