中心体蛋白Nlp对卵巢癌细胞侵袭与黏附能力的影响

Effects of Nlp on invasiveness and adherence of ovarian cancer cells

目的:探讨中心体蛋白Nlp是否促进卵巢癌细胞增殖、黏附与侵袭。方法:利用转染技术构建Nlp高表达的卵巢癌SKOV3细胞系,采用MTT法、肿瘤细胞黏附、跨膜迁移分析和平板集落形成率检测,比较SKOV3、SKOV3/GFP和SKOV3/Nlp细胞系在增殖、黏附与侵袭能力方面的差异。结果:与对照细胞相比,SKOV3/Nlp细胞的生长加快,SKOV3/Nlp细胞克隆形成率显著上升(P<0.05)。SKOV3/Nlp细胞在30min及60min时黏附率均显著大于SKOV3/GFP及SKOV3细胞(P<0.01)。SKOV3/Nlp、SKOV3/GFP、SKOV3细胞迁移至膜下的数目分别为:640±69,316±25,314±37。与SKOV3/GFP、SK-OV3细胞相比,SKOV3/Nlp细胞的迁移能力显著增强(P<0.05)。结论:当Nlp表达增高后,会导致肿瘤细胞的增殖、黏附、侵袭等恶性表型增加,提示Nlp具有促进肿瘤发生发展的生物学作用。

Objective：To investigate whether the centrosome protein Nip plays a role in the proliferation, adhesion and invasion of ovarian cancer cell. Method：To construct high-Nip- expressed ovarian cancer cell line SKOV3 with transfection technique and compare the prolifer- ation, adhesion and invasion of SKOV3/Nlp cell line with SKOV3 and SKOV3/GFP cell lines by means of MTT assay, analyses of cell adhesion, migration and colony formation. Result： Com- pared with control cells, SKOV3/Nlp cells grew faster and clone formation rate of SKOVS/Nlp greatly increased,which was significantly different by the statistical analysis（ P〈0.05 ）. Adhe- sion rate of SKOV3/Nlp was higher than SKOV3/GFP and SKOV3 cells at the moment of 30 minutes and 60 minutes, which was statistically significant（P〈0.05 ）. The number of SKOV3/ Nip, SKOV3/GFP, and SKOV3 cells migrated beneath the membrane was respectively 640±69, 316±25,314±37. Compared with SKOV3/GFP, SKOV3 cells, SKOV3/Nlp cell migration ability was stronger, which was significantly different by the statistical analysis （P 〈0.05 ）. Conclu- sion：Overexpression of Nlp expression results in the increase of tumor cell proliferation, adhe- sion, invasion and other malignant phenotypes, which prove that Nlp is an important protein in the development of tumor malignancy.