食管拉网细胞端粒酶活性检测及临床应用研究

The Clinical Application Study of the Detection of Telomerase Activity in Esophageal Netting Cells

目的 :检测食管拉网细胞中端粒酶活性 ,探讨其在食管癌诊断及预测预后的临床应用价值。方法 :应用端粒重复序列扩增法 ,对 30例食管拉网细胞标本及相应的癌组织和癌旁正常食管组织进行检测。结果 :30例食管拉网标本中端粒酶活性阳性率为 83.3% ( 2 5/ 30 ) ,相应癌组织中阳性率为 96 7%( 2 9/ 30 ) ,14例正常食管组织中阳性率为 14.3% ( 2 / 14)。拉网细胞和癌组织中端粒酶阳性率与正常组织相比较差异有显著性 (P <0 .0 0 5) ;食管拉网细胞端粒酶活性阳性率高于拉网细胞学癌细胞检出率( 73.3% ) ,但差异无显著性 (P >0 .0 5) ;端粒酶活性与食管癌临床病理因素无关。结论

Objective:To detect the telomerase activity in esophageal netting cells and explore its clinical application value in the diagnosis of esophageal carcinoma.Methods: Telomerase activity was detected by Telomeric Repeat Amplification Protocol(TRAP) assay in 30 cases of the matched tumor tissues and 14 cases of tumor adjacent tissues.Results:The positive rate of telomerase activity was 83.3% in the esophageal netting cells(25/30),96.7% in the matched tumor tissues(29/30) and 14.3% in the tumor adjacent tissues(2/14) respectively. The positive rate of telomerase activity in the netting cells and the matched tissuses was significantly higher than that in the tumor adjacent tissuses(P<0.005).The positive rate of telomerase activity is higher than that of cytology in the esophageal netting cells(83.3% to 73.3%),but the difference was not significant (P>0.05). There was no significant relation between the telomerase activity and the clinicopathological factors of esophageal carcinoma.Conclusions:Detection of telomerase activity combination with cytology in esophageal netting cells could improve the positive rate of earlier diagnosis of esophageal carcinoma.