神经生长因子对醋酸铅毒性拮抗效应的研究

Experiment Investigation on the Effects of Nerve Growth Factor Against the Neurotoxicity of Lead Acetate

目的 :研究神经生长因子 (NGF 2 .5S)对不同浓度的醋酸铅神经毒性拮抗效应 ,观察细胞分化以及细胞形态的影响 ,同时进行了醋酸铅的毒性试验和细胞分化的比较。方法 :利用大鼠胚胎中脑神经细胞作原代微团培养。观察不同浓度 ( 0 .0 0 1～ 5 0 μmol/L)醋酸铅对中脑细胞生长发育的影响。 结果 :染毒组的细胞集落数形成率明显降低 ,细胞体积小 ,细胞间神经纤维减少 ,表明醋酸铅可抑制胚胎中脑神经细胞的分化 ,其半数分化抑制浓度为 1 .0 μmol/L ,半数增殖抑制浓度为 2 0 .0 μmol/L ,并显剂量 效应关系。当加入NGF 2 .5S后 ,在同浓度的醋酸铅情况下 ,微团中的集落形成率有明显的增加。结论 :NGF 2 .5S对醋酸铅的神经毒性有一定的拮抗效应。

Purpose:To investigate the antagonistic effect of nerve growth factor (NGF 2.5S) on the neurotoxicity of lead acetate. Methods:By means of the micromass culture from rat embyo midbrain, the morphologic changes of the neurons in the micromass were observed and the rates of colony formation were calculated. Results:There were higher rates of colony formation and degree of cell differentiation in the NGF 2.5S lead acetate group than those in the lead acetate group. Conclusion:NGF 2.5S can antagonize the neurotoxicity of lead acetate.