曲格列酮对大鼠垂体腺瘤GH3细胞CD147、TGF-β1和MMP-9表达的影响

Effects of troglitazone on the expressions of CD147, TGF-β1 and MMP-9 in GH3 cells

目的探讨过氧化物酶体增殖激活物受体γ(PPAR-γ)的配体曲格列酮(TGZ)对大鼠垂体腺瘤GH3细胞系白细胞分化抗原147(CD147)、转化生长因子β1(TGF-β1)和基质金属蛋白酶9(MMP-9)表达的影响。方法GH3细胞分为空白对照组和实验组(TGZ浓度分别为1×10-3、1×10-4和1×10-5mol/L)。TGZ作用GH3细胞48 h后,分别采用Western blot和荧光定量PCR检测各组细胞中CD147、TGF-β1、MMP-9蛋白和mRNA的表达。结果各组细胞CD147、TGF-β1、MMP-9蛋白和mRNA的表达与空白对照组相比,均有统计学差异(P均<0.05),而且TGZ浓度越高,CD147、TGF-β1、MMP-9蛋白和mRNA的表达量越低。结论 PPAR-γ配体TGZ能降低CD147、TGF-β1、MMP-9的表达,并可能通过该作用减弱GH3细胞的侵袭力。

Objective To explore the effect of troglitazone（TGZ）, a ligand for peroxisome proliferator-activated receptor γ（PPAR-γ）, on the expressions of cluster of differentiation 147（CD147）, transforming growth factor beta 1（TGF-β1） and matrix metalloproteinase 9（MMP-9） in rat GH-secreting GH3 cells. Methods GH3 cells were devided into control group and experimental groups （1×10-3, 1×10-4 and 1×10-5 mol/L TGZ）. After treated with TG2 for 48 hours, expressions of CD147, TGF-β1 and MMP-9 at protein and mRNA levels were detected by Western blot and Real-time PCR respectively. Results The expressions of CD147, TGF-β1 and MMP-9 at protein and mRNA levels in all the TGZ treated groups showed significant differecnces compared with control group（all P〈0.05）. Moreover, expressions of the above three indicators decreased both at protein and mRNA levels when TGZ concentration was increased. Conclusion TGZ can inhibit expressions of CD147, TGF-β1 and MMP-9, which might relieve the invasiveness of GH3 cells.