乳腺癌化学治疗敏感与耐药组织相关蛋白表达差异

Differential protein expressions in breast cancer between drug sensitive tissues and drug resistant tissues

目的:研究乳腺癌化学治疗(化疗)敏感及耐药相关蛋白。方法:根据新辅助化疗效果筛选出化疗敏感与耐药组,应用蛋白质组学技术研究两组间的差异蛋白并采用蛋白质印迹方法对部分差异蛋白进行验证。结果:在化疗敏感组及耐药组乳腺癌组织蛋白质谱中找到13种差异蛋白质,其中3种在耐药组上调,10种下调。对其中7种蛋白质进行验证,角蛋白I型细胞骨架19(keratin type I cytoskeletal 19,KIC19)、胸苷磷酸化酶(thymidinephosphorylase,TYPH)在耐药组表达上调,而热休克蛋白27(heat shock protein 27,HSP27)、角蛋白I型细胞骨架9(keratin type I cytoskeletal 9,KIC9)、胶原蛋白α-2(六)链[collagen alpha-2(VI),CO6A2]、波形蛋白(vimentin,VIME)、β肌动蛋白(actin cytoplasmic 1,ACTB)等表达下调,差异有统计学意义(P<0.01)。结论:KIC19和TYPH可能与乳腺癌化疗耐药相关,而HSP27,KIC9,CO6A2,VIME以及ACTB可能与化疗敏感相关。

Objective： To investigate the differential expression of the sensitive and resistant relative proteins in human breast cancer tissue. Methods： A drug sensitive group and a drug resistant group for chemotherapy in patients with breast cancer were selected through neoadjuvant. The differential protein expression in 2 groups was detected by proteomics techniques, and parts of differential proteins were identified by Western blot. Results： There were 13 differential proteins in the 2 groups, in which the expression of 3 proteins was up-regulated and 10 down-regulated. Seven proteins were identified by Western blot. The expression of keratin type I cytoskeletal 19 （KIC19）, thymidine phosphorylase （TYPH） was upregulated, and the expression of heat shock protein 27 （HSP27）, keratin type I cytoskeletal 9 （KIC9）, collagen alpha-2（VI） （CO6A2）, vimentin （VIME）, and actin cytoplasmic 1 （ACTB） was down-regulated in the drug resistant group. Xhere was significant difference between the 2 groups （P〈0.01）. Conclusion： The expression of KIC19 and TYPH may be correlated with drug resistance in patients with breast cancer, and HSP27, KIC9, CO6A2, VIME, and ACTB may be correlated with drug sensitivity.