中药复方“冬夏丸”体外抑制Epstein-Barr病毒抗原表达的研究

Inhibition of the expression of Epstein Barr virus antigens in vitro by Chinese medicine compound “Dongxia wan”

目的 研究中药复方“冬夏丸”体外对Epstein Barr病毒 (EBV)抗原表达的影响。方法应用细胞培养和间接免疫酶技术。结果 2 0～ 80 μg/ml中药复方“冬夏丸”与靶细胞共培养能显著的抑制巴豆油和正丁酸联合诱发Raji细胞的EBV早期抗原 (EBV EA)和B95 8细胞的EBV壳抗原 (EBV VCA)表达及B95 8细胞的EBV VCA自然表达 ,抑制率分别是 19 31%～ 49 84% ,34 6 3%～ 45 6 1%和2 1 6 7%～ 47 78%。用该药预处理Raji和B95 8细胞亦能显著地抑制巴豆油和正丁酸联合诱发EBV EA和VCA的表达。结论 中药复方“冬夏丸”具有明显抑制EBV抗原表达的作用 ,有可能用于EBV相关疾病的防治。

Objective To investigate the effect of Chinese medicine “Dongxia wan” on the expression of Epstein Barr virus (EBV) antigens in vitro.Methods Cell culture and indirect immunoenzyme methods were used.Results When Raji and B95 8 cells were cultured with 20- 80 μg/ml of “Dongxia wan”, the expression of EBV early antigen (EBV EA) and EBV capsid antigen (EBV VCA) induced by croton oil and n butyric acid and the natural expression of EBV VCA were all inhibited significantly. The inhibition rates were 19 3%-49 84%, 34 63%-45 61% and 21 67%-47 78% respectively. When Raji and B95 8 cells were pretreated with “Dongxia wan” the expression of EBV EA and EBV VCA were also inhibited obviously.Conclusion Chinese medicine “Dongxia wan” can inhibit the expression of EBV antigens in target cells, it may be used to the prevention and treatment of EBV related diseases.