酶联免疫吸附法检测庚型肝炎病毒抗体

Detection of IgG antibody to hepatitis G virus by ELISA

目的 探讨抗庚型肝炎病毒 (HGV)IgG与各种病毒感染、丙氨酸转氨酶 (ALT)及HGVRNA的相关性。方法 用酶联免疫吸附试验 (ELISA)法检测了 315例各种肝炎病毒 (A～E)感染者和117例健康献血员血清的抗 HGVIgG ,并测定其ALT ,对抗 HGVIgG阳性的标本用逆转录 聚合酶链反应 (RT PCR)法检测HGVRNA。结果 献血员抗 HGVIgG的阳性率为 3.42 % (4/117) ,非A～E、HAV、HBV、HCV、HEV、HBV和HCV、HBV和HDV、HBV和HEV病毒感染者抗 HGVIgG阳性率分别为 5 .95 %(5 /84) ,0 (0 /3) ,3.75 % (3/80 ) ,17.2 8% (14/81) ,0 (0 /15 ) ,33.33% (5 /15 ) ,3.33% (1/30 ) ,16 .6 6 % (1/6 ) ;抗 HGVIgG和HGVRNA的相关性为 30 .30 % (10 /33) ,抗 HGVIgG阳性标本中ALT升高率为 33.33%(11/33)。结论 HGV可以单独或 /和HBV、HCV合并感染机体且与HCV密切相关 ,HGV和HCV合并感染将增加肝脏的损伤程度。

Objective To study the relation of HGV IgG antibody (anti HGV IgG) to various viral infections, ALT and HGV RNA.Methods To detect anti HGV IgG from 315 persons infected with various viruses hepatitis (A～E) and 117 healthy blood donors by ELISA. The detection of ALT of these peoples, and detect HGV RNA of the samples of positive anti HGV IgG by RT PCR were also carried out.Results The positive rate of anti HGV IgG of 117 blood donors was 3.42%(4/117),and that of the people infected by non A～E,HAV,HBV,HCV,HEV,HBV and HCV, HBV and HDV, HBV and HEV were 5.95%(5/84),0(0/3),3.75%(3/80),17.28%(14/81),0(0/15),33.33%(5/15),3.33%(1/30),16.66%(1/6), respectively. The relativity of anti HGV IgG and HGV RNA was 30.30%(10/33), and 33.33% of ALT level in the antibody positive samples were elevated.Conclusion HGV may infect people alone, or coinfect with HBV or /and HCV, but is highly relative to HCV, and the coinfection of HGV with HCV may increase the degree of liver damage.