肝病患者庚型肝炎病毒感染的研究

The study on hepatitis G vrus infection among patients with liver diseases

目的 研究肝病患者庚型肝炎病毒感染状况。方法 用酶链免疫吸附试验 (ELISA)检测 15 4例门诊和住院肝病患者 (均无输血史 )的抗 HGV ,15 4例中有 5 4例同时采用逆转录聚合酶链反应 (RT PCR)检测HGVRNA ,分别计算抗 HGV和HGVRNA的阳性率。以HGVRNA阳性为HGV感染的判断标准 ,来分析各类肝病患者HGV感染状况。结果 15 4例肝病患者抗 HGV阳性者 31例 ,阳性率 2 0 13% ;5 4例检测HGVRNA者阳性 13例 ,阳性率 2 4 0 7%。慢性肝炎和脂肪肝患者HGVRNA的阳性率 (比 )分别为 2 0 0 0 % (7/35 )和 5 /6。结论 (1)HGV感染在非经血途径传播的散发性肝炎患者中 ,也有相当程度的流行 ;(2 )HGV是慢性肝炎的致病因子之一 ;(3)在脂肪肝患者中检出HGVRNA的临床意义有待进一步研究。

Objective To investigate the hepatitis G virus(HGV) infection among patients with liver diseases.Methods Anti HGV was detected by ELISA among 154 patients with liver diseases (no history of blood transfusion), 54 of them, HGV RNA was detected by RT PCR concomitantly. The rates of anti HGV and HGV RNA were calculated, respectively. The positivity of HGV RNA was taken as diagnostic criteria for HGV infection in different kinds of liver disease.Results Of 154 patients with liver diseases, anti HGV was positive in 31 patients, the detectable rate of anti HGV was 20 13%.HGV RNA was detected in 13 of 54 patients, the detectable rate was 24 07%. The positive rates of HGV RNA tested in 35 patients with chronic hepatitis and 6 patients with fatty liver were 20 00%(7/35) and a ratio of 5/6, respectively.Conclusion (1)HGV infection is considerably prevalent among patients with nontransfusion transmitted sporadic hepatitis. (2)HGV is a responsible agent for chronic hepatitis. (3)The clinical significance of detectable HGV RNA in patients with fatty liver remains for further study.