慢性肝炎和原发性肝癌肝组织P21和P15蛋白的表达及与细胞凋亡的关系

The expression of P21 and P15 proteins in liver tissue of chronic viral hepatitis and hepatocellular carcinoma and their relation to cell apoptosis

目的 研究P2 1和P15蛋白在慢性肝炎、原发性肝癌肝组织中的表达及与肝细胞凋亡的关系。方法 对 36例慢性肝炎、10例原发性肝癌肝组织进行P2 1和P15蛋白免疫组化及肝组织DNA损伤检测。结果 P2 1和P15蛋白在慢性病毒性肝炎 (CH)肝组织中的表达主要为肝细胞核阳性 ,而原发性肝癌则均表达在癌细胞的胞质中 ,在细胞核中无表达 ;中重度CH(G3～G4)患者P2 1、P15蛋白的表达水平显著高于轻度 (G1～G2 )患者 ,与CH肝组织DNA损伤的相关系数分别为 0 5 6、0 5 1(P <0 0 1)。结论 P2 1和P15蛋白参与了CH肝组织的病理损伤及肝细胞凋亡。

Objective To study the expression of P21 and P15 proteins in liver tissue of chronic viral hepatitis (CH) and hepatocellular carcinoma (HCC) and their relation to cell apoptosis.Methods The expression of P21 and P15 proteins in 36 CH patients liver tisues' were detected by immunohistochemistry and the DNA damage of the hepatocytes were detected by TDT mediated dUTP nick and labeling(TUNEL). 10 cases of HCC were also studied.Results P21 and P15 proteins mainly distributed in the interstitium of portal areas,the piece meal necrotic sites and the nuclei and cytoplasm of the hepatocytes.The higher expression of P21 and P15 proteins in the interstitium and nuclei of the hepatocytes was seen in CH as compared with that in HCC. The levels of expression of P21 and P15 in moderate and severe type CH were higher with that of the mild type CH (P<0 05). The correlative coefficient of expression of P21 and P15 with the DNA damage of hepatocytes were 0 56, 0 51 respectively (P<0 01).Conclusion The enhanced expression of P21 and P15 proteins in CH correlated with the injury of liver tissues and apoptosis of hepatocytes.