从烟台地区蚊虫分离到披膜甲属虫媒病毒

The arboviruses of genus alphavirus family Togavirdae were isolated from mosquites captured from Yantai

目的 从烟台地区自然界捕捉的蚊虫中分离虫媒病毒。方法 对分离的 15株虫媒病毒中的 3株进行理化特性分析 ,电镜检查以及特异性免疫反应观察。结果 多次实验均显示该病毒对乙醚敏感 ,抵抗 5′ 碘脱氧尿苷 (5′ Idu) ,能在白蚊伊蚊C6 / 36传代细胞上生长繁殖 ,并能引起典型细胞病变 ,乳鼠脑内接种不引起规律发病和死亡。电镜观察 ,可见大小相同的球形病毒颗粒 ,毒粒直径约 (5 5± 2 3)nm。该病毒与乙脑病毒和布尼亚病毒科的组特异性免疫腹水均不反应 ,而与披膜病毒科甲属特异性免疫腹水有交叉反应。结论 本次分离到的病毒为披膜甲属虫媒病毒。

Objective To isolate arbovirus from mosquitoes caught from Yantai.Methods The isolated viruses were tested for their physico chemical properteis,examined by electron microcopy and specific immuno reactivity.Results Fifteen strains of virus were isolated from mosqiutes in 1994 from Yantai. Three of them were further assayed. The results showed that the viruses could multiply on C6/36 cell and produce typical cytopathogenic effect. The viruses couldn′t cause regular sickness and death of suckling mice by intracerebral inoculation. The viruses were sensitive to ether, but resistant to 5′ Idu. One sample was exmained with electron microscope, and spherical virus particles were observed. The diameter of the virus particles is about 55±2 3nm. It did not react with the JBE virus and Bunyaviridae group specific immuno ascitic fluids but cross reacted with the group A Togaviridae specific immuno ascitic fluid.Conclusion The results showed that the viruses isolated fom Yantai belong to Alphavirus of Togaviridae.