不同强度微波辐射对小鼠脑组织钙、镁ATP酶活性的影响

Effect of microwave irradiation of different densities on Ca^(2+),Mg^(2+)-ATPase activity of mice brain

目的 探讨微波对机体中枢神经系统作用的机理。 方法 用 2 45 0 MHz连续波微波理疗机为辐射源 ,以昆明小鼠为对象 ,观察在 1m W/ cm2、5 m W/ cm2和 10 m W/ cm2三种照射强度下 ,小鼠脑皮层、海马及丘脑细胞膜 Ca2 +、Mg2 + - ATP酶活性的变化情况 ,用组织化学方法测定其活性。 结果 1m W/ cm2时 ,皮层、海马及丘脑 Ca2 +、Mg2 + - ATP酶活性均比对照组高 (P<0 .0 5 ) ,5 m W/ cm2 时 ,酶活性与对照组差别均无显著性的意义 ,10 m W/ cm2 时 ,皮层、海马酶活性比对照组显著降低 ,而丘脑则无明显变化。 结论 2 45 0 MHz微波照射小鼠时 ,脑组织细胞膜上 Ca2 + 、Mg2 + -ATP酶活性随照射强度不同而有不同变化。受高强度照射时 ,参与学习记忆功能的皮层。

Objective To investigate the mechanism of effect of microwave irradiation on central nervous system. Methods The cell membrane Ca 2+ ,Mg 2+ ATPass activities of cerebral cortex,hippocampus,thalamus of Kunming mice exposed to 1 mW/cm 2,5 mW/cm 2,10 mW/cm 2 microwave irradiation were measured by histochemical method. Results In rats underwent 1 mW/cm 2 exposure,the activities of Ca 2+ ,Mg 2+ ATPase of cerebral cortex,hippocampus,as wall as thalamus increased significantly ( P <0.05) as compared with controls .In rats underwent 5 mW/cm 2 exposure,Ca 2+ ,Mg 2+ ATPase of the above 3 parts of brain were not changed obviously,while in 10 mW/cm 2 group, Ca 2+ ,Mg 2+ ATPase activities in cerebral cortex and hippocampus were significantly decreased ,and those of thalamus were not changed significantly. Conclusion Dfferent densities of microwave irradiation may result in diffent reaction of Ca 2+ ,Mg 2+ ATPase of brain. Under 10 mW/cm 2 exposure, negative effect on cerebral cortex and hippocampus came earlier than effect on thalamus. This implies that the learning ability and memory were affected earlier than on thermal regulation under high density microwave irradiation.