双球囊导管法基因导入血管的实验研究

Experimental study on gene transfer into vessel using double-balloon catheter

目的 探讨双球囊导管法腺病毒介导性基因导入犬股动脉的效率及时间曲线。方法 选成年杂交犬 2 0只 ,经股动脉分支逆行插入 7F双球囊导管至髂动脉 ,采用气囊法剥脱血管内皮后 ,充盈双球囊 ,经球囊间侧孔注入 0 3mL约含 3 0× 10 9pfu的含有细菌lacZ基因的重组腺病毒入间腔 ,培养 30分钟。术后 1、3、7、14、2 1、2 8及 35天处死动物 ,组织化学分析法检测lacZ基因在股动脉的表达。结果 导入基因后 2 4小时即见lacZ基因在血管内膜表达 ,7天达高峰 ,持续 4周左右。高峰时 ,培养间腔血管内侧近 10 0 %表达了外源基因 ,深度达血管中膜 2 /3。结论 双球囊导管法腺病毒介导性基因导入犬股动脉呈高效及一过性特点。

Objective To evaluate the efficiency and time curve of gene expression following adenovirus-mediated gene transfer into canine injured femoral arteries through double balloon catheter. Methods Femoral arteries were first subjected to the balloon injury and 3.0×10 9 pfu of adenovirus expressing bacterial lacZ gene was filled into a closed space created by inflated double-balloon catheter, subsequently incubated for 30 min. The vessel were harvested at 1,3,7,14,21,28 and 35 days after gene transfer.Results The lacZ expression was found in 100% of arterial inner surface and in two-third of arterial media, which detected at 1 day, peaked at 7 days and lasted up to 28 days.Conclusion This study indicated that adenovirus mediated gene transfer into arteries was transient but high efficient.