肝心宁对肝纤维化大鼠肝组织病理及PDGF-BB、TGF-β、MMP-1的影响

Effects of Ganxinning Capsule on the Liver Tissue Pathology and PDGF-BB,TGF-β1, MMP-1 Factors in HF Rats

目的:从肝纤维化的病理学和血清标志物方面,探讨肝心宁对肝纤维化大鼠肝组织病理的影响。方法:将60只SD大鼠随机分成正常对照组,肝纤维化模型组和肝心宁干预纤维化组。采用改良式复合因素法复制大鼠肝纤维化模型。正常对照组与肝纤维化模型组给予生理盐水10mL/kg灌胃,肝心宁干预纤维化组给予10g/kg治疗。6周后,取各组大鼠肝脏组织,HE染色比较各组大鼠肝脏组织的病理学变化,检测比较不同组大鼠血清中肝纤维化指示物血小板生长因子-BB(PDGF-BB)、转化生长因子-β1(TGF-β)、基质蛋白酶-1(MMP-1)的含量。结果:肝组织病理学:与肝纤维化模型组相比,干预组炎症坏程度减轻,肝纤维化程度明显改善,血清PDGF-BB、TGF-β1含量显著下降,而MMP-1含量显著升高。结论:肝心宁能有效改善肝纤维化血清学指标和病理学指标。

Objective： To study the effects of Ganxinning Capsule on hepatic fibrosis （HF） rats from the pathological aspect and serum hepatic fibrosis markers. Methods： 60 rats were randomly divided into the normal group, HF group, Ganxinning Capsule treated group. The HF model in rats was duplicated with Improved Composite Factor Method. Rats of the normal control group and the HF group were administered physiological saline with dose 10mL/kg, the Ganxingning Capsule treated rats were administered Ganxinning with the does 10g/kg. The live pathology change and the level of serum hepatic fibrosis markers of each group were compared 6 weeks later. Results： Compared with the HF rats, the hepatic inflammatory necrosis and the hepatic fibrosis degree in the Ganxinning Capsule treated rats were significantly rescued （P 〈0.05）. besides that, the serum levels of PDGF-BB and TGF-β1 were obviously lower, the serum MMP-1 was obviously higher. Conclusion： Ganxinning Capsule could obviously improve serological indexes and pathological indexes of HF.