口底鳞癌患者血管内皮生长因子受体和Periostin的检测及其临床意义

The expressions of vascular endothelial growth factor receptor and Periostin in floor-of-mouth squamous cancer and its clinical significance

目的通过检测口底鳞癌患者癌组织中血管内皮生长因子受体（VEGFR）和Pefiostin的表达水平，探讨其相关的临床意义。方法选取口底鳞癌患者手术切除癌组织标本64例（观察组），每例另取手术切除癌组织旁非癌组织标本（对照组）。采用荧光定量PCR检测VEGFR和Pefiosfin mRNA的表达量。结果观察组VEGFR和Pefiostin mRNA的表达量分别为0．514±0．142、0．502±0．125，显著高于对照组的0．204±0．086、0．184±0．064，两组比较差异有统计学意义（P〈0．01）。VEGFR和Pefiostin mRNA的表达量与性别无相关性（P〉0．05），但与肿瘤分化程度、淋巴结转移情况、临床分期显著相关（P〈0．05）。相关性分析显示口底鳞癌组织中VEGFR和Pefiosfin mRNA的表达呈显著正相关（r=0．624，P〈0．01）。结论VEGFR和Pefiostin在口底鳞癌的发生、发展中起到一定的作用，联合检测对于口底鳞癌的诊断、分级以及预后评估具有一定的临床指导意义。

Objective To investigate the mRNA expressions of vascular endothelial growth factor receptor （VEGFR） and Periostin in floor-of-mouth squamous cancer and explore its clinical significance. Methods Cancer tissues of 64 floor-of-mouth squamous cancer patients were enrolled as observation group, and the adjacent normal tissues were collected as control group. The mRNA expressions of VEGFR and Periostin were detected by fluorescent quantitation PCR analysis. Results The mRNA expressions of VEGFR and Periostin in observation group were significantly higher than those in control group （0.514 ± 0.142 vs. 0.204 ±0.086 and 0.502 ±0.125 vs. 0.184 ± 0.064,P 〈 0.01 ）. The mRNA expressions of VEGFR and Periosfin had no significantly related with gender,and they were significantly related with histological grade,lymphatic metastasis and clinical pathologic stage （P 〈 0.05）. The mRNA expression of VEGFR was significantly positive related with the mRNA expressions of Periostin in floor-of-mouth squamous cancer tissues （r =0.624,P 〈0.01）. Conclusions VEGFR and Periostin maybe play a key role in the development of floor-of-mouth squamous cancer,and be used as the markers for estimating the degree of differentiation of floor-of-mouth squamous cancer.