EZH2在原发性肝癌中的表达及与分化和增殖的关系

Expression of EZH2 and its relation to differentiation and proliferation in hepatocellular carcinoma

目的:探讨EZH2的表达水平与原发性肝癌(HCC)的恶性程度和增殖能力的关系及其意义。方法:采用HE染色判断HCC的分化级别;免疫组化SP法检测EZH2与Ki67蛋白在不同分化级别HCC组织中的表达水平。结果:EZH2在HCC组织中的表达率显著高于正常肝组织(P<0.05);EZH2的表达水平随着HCC分化程度的降低而明显升高(P<0.05);EZH2与Ki67蛋白在不同分化级别的HCC中表达趋势一致。结论:HCC中EZH2蛋白的表达水平显著升高,与肿瘤的分化程度和增殖相关,提示EZH2可为判断HCC病理分级和增殖能力提供参考。

Objective： To study the expression of EZH2 gene in hepatocellular carcinoma （ HCC）, and investigate the relationship between EZH2 gene and the differentiation and proliferation of HCC and pathological significance. Methods： HE staining was used to measure the level of differentiation of HCC, immunohistochemistry were used to de- tect the expression of EZH2 and Ki67 protein in HCC tissues. Results：The expression of EZI-I2 in HCC significantly increased than normal hepatic tissue （P 〈 0.05 ）, with decreasing of the differentiated degree, the positive expression of EZH2 protein was significantly higher in HCC （ P 〈 0.05 ）. The trends of positive expression of EZH2 and Ki67 protein had consistency. Conclusion： Increased expression of EZH2 was shown in HCC and related to tumor differenti- ation degree and proliferation, it may be valuable for pathological diagnosis.