男性乳腺癌血清VEGF水平与外周血循环肿瘤细胞的相关性及临床意义

Level of serum VEGF in male breast cancer and its association with circulating tumor cells

目的:检测男性乳腺癌患者血清中VEGF及外周血CK19的表达水平,探讨二者的相互关系及临床意义。方法:应用ELISA法分别检测18例男性乳腺癌患者及8例健康男性志愿者血清中VEGF的水平,同时抽取相应的外周血标本,应用实时定量逆转录-聚合酶链反应(qRT-PCR)方法检测标本中CK19的表达水平,并进行相关性分析。结果:VEGF在男性乳腺癌患者血清中的表达水平明显高于正常健康男性,二者差异显著(P=0.015);亚组分析表明,在腋淋巴结阳性的患者中,血清VEGF水平明显高于淋巴结阴性患者(P=0.026)。CK19在健康男性外周血标本中未见表达,在男性乳腺癌患者外周血中,阳性表达5例(27.8%),而外周血中CK19阳性的男性乳腺癌患者血清VEGF水平显著高于CK19阴性的患者(130.34±77.18pg/ml vs58.01±52.43pg/ml,P=0.038)。结论:男性乳腺癌患者血清VEGF水平与外周血微转移密切相关,提示VEGF可能参与了肿瘤细胞的侵袭和播散,可作为男性乳腺癌早期血行播散的血清学指标。

Objective：To explore the level of serum VEGF in male breast cancer and its clinical significance as well as the relationship with peripheral blood circulating tumor cells in the patients. Methods：Level of serum VEGF in 8 cases of healthy male volunteer and 18 cases of breast cancer were detected by ELISA. CK19 mRNA in peripheral blood was detected by qRT - PCR as an index of circulating tumor cells in the patients as well. Corretation between serum VEGF level and peripheral blood circulating tumor cells was analyzed. Results： Level of serum VEGF in male breast cancer were significantly higher compared with those in healthy volunteer （ P = 0.015 ）. A subgroup analysis showed that in lymph node positive patients, level of serum VEGF were higher （P = 0.026）. CK19 mRNA was found in peripheral blood of 5 cases of male breast cancer and the positive rate was 27.8%. However,it was not detected in that of healthy volunteers. Mean level of serum VEGF in CK19 positive and negative patients was 130.34 ＋77.18 pg/ ml and 58.01 _＋52.43pg/ml,respectively. There was a significant difference between the two groups （P = 0. 038 ）. Conclusion：Level of serum VEGF in male breast cancer was closely related with the peripheral blood circulating tumor ceils. High level of serum VEGF may be a soluble biomarker for the early micrometastasis of male breast