子宫内膜癌中PTEN蛋白、P-AKT蛋白的表达及意义

PTEN protein expression and significance of P-AKTprotein in endometrial carcinoma

目的:检测子宫内膜癌组织中PTEN蛋白及P-AKT蛋白表达并探讨其意义。方法:采用免疫组织化学法检测66例子宫内膜癌、28例正常子宫内膜组织中PTEN蛋白及P-AKT的表达。结果:子宫内膜癌组织中PTEN蛋白为缺失表达,高于正常子宫内膜组织(P<0.05),而且PTEN表达在G1级肿瘤高于G2、G3级(P<0.05),PTEN蛋白缺失率与肿瘤组织类型有关(P<0.05),与肌层浸润、淋巴结转移及病理分期无明显关系(P>0.05)。子宫内膜癌组织中P-AKT蛋白表达为高表达,P-AKT的表达与肿瘤组织学分级、病理学分期、有无淋巴结转移以及肿瘤浸润子宫肌层深度显著相关(P<0.05),其表达与组织学类型无关(P>0.05)。P-AKT与PTEN的表达呈明显负相关。结论:PTEN表达缺失与临床病理参数无关,蛋白表达缺失常发生细胞分化较差的子宫内膜癌。PTEN蛋白表达缺失与P-AKT表达水平有关。

Objective：To detect endometrial carcinoma PTEN protein and the expression of P -AKT protein and study its significance. Methods： Immunohistochemical assay was used for the detection of PTEN protein and P - AKT in 66 cases of endometrial carcinoma, 28 cases of normal endometrial tissue. Results： Endometrial carcinoma tissue in PTEN protein expression was loss, higher than that in normal endometrial tissue （P 〈 0.05 ）, and the PTEN expres- sion in G~ level was higher than that of G2, G3 tumor（P 〈 0.05 ）, PTEN protein loss rate and tumor tissue types （P 〈 0.05 ） , and myometrial invasion, lymph node metastasis and pathological staging had no significant relationship （ P 〉 0. 05 ）. Endometrial carcinoma expression of P - AKT protein expression, the expression of P - AKT and tumor histo- logical grade, pathological stage, without lymph node metastasis and tumor infiltrating the myometrium depth were sig- nificantly related（ P 〈 0.05 ）, and its expression was associated with histologic types（ P 〉 0.05 ）. P- AKT and FFEN expression was significantly negative correlated. Conclusion： PTEN expression and clinical pathology parameters are unrelated with protein loss. Expression loss of PTEN protein is related with the expression levels of P - AKT.