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应用重组杆状病毒表达猪瘟病毒糖基化E0(E^(rns))蛋白 被引量：4

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摘要 通过PCR方法扩增出包含糖基化信号肽的猪瘟兔化弱毒疫苗株E0(Erns)蛋白基因,末端引入6×His标签序列,将其克隆到Bac-to-Bac杆状病毒表达系统pFastBacTMHT B载体中,将阳性重组质粒pF-E0转化DH10Bac感受态大肠杆菌,经蓝白斑筛选和PCR鉴定,获得重组Bacmid质粒(rBac-E0)。转染对数生长期的Sf9昆虫细胞获得重组杆状病毒rAc-E0。经Western blot鉴定重组蛋白正确表达,且具有良好的抗原性;用糖苷酶PNGase F处理后重组蛋白分子量减小为32 ku,表明重组蛋白为糖基化蛋白。这为进一步研究E0蛋白的功能、亚单位疫苗和诊断试剂的开发奠定了基础。
作者 李文良 毛立 张纹纹 江杰元
机构地区 江苏省农业科学院兽医研究所/农业部兽用生物制品工程技术重点实验室/国家兽用生物制品工程技术研究中心
出处 《畜牧与兽医》 北大核心 2013年第3期50-53,共4页 Animal Husbandry & Veterinary Medicine
基金 江苏省农业科技自主创新资金项目(SCX(12)5056)
关键词 猪瘟病毒 E0 重组杆状病毒 糖基化
分类号 S855.3 [农业科学—临床兽医学]
  • 相关文献

参考文献14

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二级参考文献51

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共引文献30

同被引文献38

  • 1查云峰,徐兴然,肖昌,余兴龙,涂长春.猪瘟病毒E2基因在昆虫细胞中的分泌表达及活性检测[J].畜牧兽医学报,2005,36(10):1100-1105. 被引量：6
  • 2高华峰,李富祥,张念祖,李华春.猪瘟病毒E^(rns)基因的克隆及原核表达[J].动物医学进展,2007,28(2):22-24. 被引量：3
  • 3刘利萍,王秀珍,毛威.姜油树脂/β-环糊精包合物的制备工艺优化和表征[J].中国药学杂志,2007,42(15):1164-1167. 被引量：7
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畜牧与兽医
2013年 第3期

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