猕猴外周血液中多潜能调控因子Oct4、Nanog和Sox2基因的表达

Expression of the pluripotency regulators Oct4,Nanog and Sox2 in peripheral blood of rhesus monkey

采用半定量RT-PCR对采自幼体(2岁以内)、亚成体(2～5岁)和成体(5岁以上)猕猴共28份外周血样品进行分析。结果显示,幼体和亚成体猕猴所有外周血样品均转录表达Oct4、Nanog和Sox2基因,大部分成体猕猴外周血样品(8/12)表达Oct4、Nanog和Sox2基因,少数样品仅表达Oct4和Sox2(2/12)或Oct4和Nanog(1/12),1份样品不表达3个基因;同一年龄组内,Sox2表达水平均显著高于Oct4和Nanog(P≤0.05),幼体组Oct4和Nanog的表达水平没有显著性差异(P>0.05),亚成体组和成体组Oct4的表达水平均显著高于Nanog(P≤0.05);在不同年龄组间,Oct4和Nanog表达水平没有显著性差异(P>0.05),幼体组Sox2表达水平显著高于成体组(P≤0.05)。结果表明,在生理条件下Oct4、Nanog和Sox2基因在不同年龄段猕猴外周血中均转录表达,不同基因表达水平存在一定差异,随年龄增加这些基因表达量有降低的趋势。

The present experiments aimed to evaluate transcription expression of the pluripotency core regulators Oct4,Nanog and Sox2 in peripheral blood of rhesus monkey using semi-quantita- tive reverse transcription polymerase chain reaction （RT-PCR）, and all samples of peripheral blood of rhesus monkeys were divided into three groups of the Young （under 2 years old）,the Sub-adult （2 to 5 years old） and the Adult （above five years old）. RT-PCR results demonstrated that Oct4, Nanog and Sox2 were positively expressed in all samples of rhesus monkeys at the young stage and the sub- adult stage and in most of samples （8/12） at the adult stage. However, few samples only expressed Oct4 ＆ Sox2 （2/12） or Oct4 ＆ Nanog （1/12）,and negatively ex- pressed the three genes in the Adult group. Comparison of these gene mRNA relative contents a- mong the samples expressing the three genes, demonstrated that Sox2 were significantly higher than Oct4 and Nanog in every group,Oct4 were significantly higher than Nanog in the sub-adult group and the adult group, and Oct4 ~ Nanog was no significant in the young group. Meanwhile, Oct4 Nanog was not significant between the three groups, and Sox2 were Significantly higher in the young group than in the adult group. The results suggested that Oct4, Nanog and Sox2 were positively ex- pressed in peripheral blood of rhesus monkeys varied from the young to the adult under the physiological condition, exhibited some differences and a decreasing or disappearing trends with age increasing.