麻疹病毒沪-191株在鸡胚成纤维细胞中连续传代的遗传稳定性

Genetic stability of measles virus Shanghai-191 strain after continuous subculture in chick embryo fibroblasts

目的研究麻疹病毒沪-191株在鸡胚成纤维细胞中连续传代的遗传稳定性。方法将麻疹病毒沪-191株原始种子批(P25)在鸡胚成纤维细胞上传代2次(P27)作为主种子批,继续传代3次(P30)作为工作种子批,继续传代至33(P33)和35(P35)代。对P25、P27、P30、P33和P35病毒基因组进行基因序列分析,并对所有代次病毒进行滴度测定。结果麻疹病毒沪-191株在鸡胚成纤维细胞中从25代传至35代,其滴度维持在5.0～5.875 lgCCID50/ml之间;P25、P27、P30、P33、P35 5个代次病毒的全基因组序列未发生任何核苷酸和氨基酸改变。结论麻疹病毒沪-191株在鸡胚成纤维细胞连续传代,病毒滴度均一,基因组序列未发生改变,遗传稳定性良好。

Objective To investigate the genetic stability of measles virus Shanghai-191 strain after continuous subculture in chick embryo fibroblasts. Methods The seeds from primary seed lot of measles virus Shanghai-191 strain （P25） strain was subcuhured in chick embryo fibroblasts for two passages （P27） and used as mater seed lot, for further three passages （P30） as working seed lot, and further subcuhured to passages 33 （P33） and 35 （P35）. The genome of various passages were sequenced, while the titers were determined. Results The titer of Shanghai-191 strain from P25 to P35 were maintained at 5.0 - 5. 875 lgCCIDso/ml. No any change of nucleotides or amino acids were observed in genomes P25, P27, P30, P33 and P35. Conclusion The titer of measles virus Shanghai-191 strain was stable after continuous subculture in chick embryo fibroblasts, while the genome sequence showed no change, indicating high genetic stability of the strain.