苏尼替尼对HeLa细胞增殖及3-磷酸甘油醛脱氢酶表达和活性的影响

Effect of sutent on proliferation of HeLa cells as well as expression and activity of glyceraldehyde-3 phosphate dehydrogenase

目的探讨苏尼替尼(Sutent)对HeLa细胞增殖及3-磷酸甘油醛脱氢酶(Glyceraldehyde-3-phosphate dehydro-genase,GAPDH)表达和活性的影响。方法用不同浓度的Sutent作用于HeLa细胞,采用MTT法检测Sutent对HeLa细胞增殖活力的影响;Western blot检测HeLa细胞中GAPDH蛋白表达的变化;GENMED细胞GAPDH活性终点比色法定量检测了GAPDH的活力。结果经Sutent处理24 h后,HeLa细胞的增殖受到明显抑制,且呈浓度依赖性,半数抑制浓度(IC50)为10.283μmol/L;Sutent可明显降低HeLa细胞中GAPDH蛋白的表达量,并能直接抑制GAPDH的活力。结论 Sutent可抑制HeLa细胞的增殖,降低GAPDH蛋白的表达量及活力,显示了其作为多靶点药物的重要研究价值。

Objective To investigate the effect of sutent on the proliferation of HeLa cells as well as the expression and activity of glyceraldehyde-3 phosphate dehydrogenase（GAPDH）. Methods HeLa cells were treated with sutent at various concentrations, of which the proliferation activity was determined by MTT method, and the expression of GAPDH by Western blot. The GAPDH activity was determined by GAPDH active terminal colorimetric analysis. Results The proliferation of HeLa cells were inhibited significantly 24 h after treatment with sutent. The inhibitory effect was dose-dependent, with a EC50 of 10. 283 μmol/L. Sutent decreased the GAPDH expression level in HeLa calls significantly and inhibited GAPDH activity directly. Conclusion Sutent inhibited the proliferation of HeLa cells and decreased the expression level and activity of GAPDH, indicating its important value as a multi-target drug.