阴道嗜酸乳酸杆菌对白念珠菌影响的体外研究

The effect of vaginal Lactobacillus acidophilus on part of virulence factors of Candida albicans

目的：研究阴道内嗜酸乳酸杆菌对白念珠菌部分毒力的影响，以进一步探讨嗜酸乳酸杆菌对白念珠菌抑制作用的机制。方法：分别采用血球仪计数法、小牛血清玻片法检测分离自阴道内的嗜酸乳酸杆菌（Lacl）对来自临床的99株白念珠菌生长及芽管生成的影响，在该基础上采用小牛血清白蛋白（BSA）平皿法和卵黄平皿法测定Lacl对白念珠菌的分泌性蛋白酶和细胞外磷脂酶活力的影响，并以ATCC4356（嗜酸乳酸杆菌）为对照。结果：白念珠菌单独存在与加入Lacl后，白念珠菌的菌数分别为（1．61±0．01）xlO。CFU／mL和（1．34±0．01）×10^8 CFU／mL；每高倍视野白念珠菌的芽管数分别为（6．17±0．17）个和（1．71±0．10）个：分泌性蛋白酶活力分别为O．5639±0，0766和0．8966±0．1058；胞外磷脂酶活力分别为0．5554±0．0778和0．8898±0．1233。P值均〈0．001，差异有统计学意义。结论：阴道内嗜酸乳酸杆菌（Lacl）对白念珠菌的生长、出芽、分泌性蛋白酶活力及细胞外磷脂酶活力均有抑制作用。

Objective： To observe the effect of vaginal L.acidophilus on part of virulence factors of Candida albicans, and ex- plore the inhibitory mechanism of Lactobacillus acidophilus to Candida albicons. Methods： Blood cell counter, calf serum slide assay were used to detect the impact of L.acidophilus （Lacl） on the growth and formation of germ tube of Candida albicons isolated from clinical vaginal secretion. Based on these, bovine serum albumin （BSA） and egg-yolk-containing plates were used to detect the activities of secretory proteinase （SAP） and the extracellular phospholipases of these Candida albicans and observe the changes of secretory proteinase and phospholipase after adding vaginal Lactobacillus acidophilus to C.albieans, and L.acidophilus （ATCC 4356） was used as a control. Results： Before and after adding Lactobacillus acidophilua, the numbers of Candida albicans were （1.61±0.01）x10s CFU/ml and （1.34±0.01）x10s CFU/ml, respectively, and the numbers of germ tubes under each high power field were 6.17±0.17 and 1.71±0.10, respectively. The activities of secretion of proteinase were 0.563 9± 0.076 6 and 0.896 6±0.105 8, and the activities of extraeellular phospholipases were 0.555 4±0.077 8 and 0.889 8±0.123 3, re- spectively （P〈 0.001）. Conclusions： Vaginal Lactobacillus acidophilus can inhibit the growth, formation of germ tube, the ac- tivities of secretory proteinase （SAP） and the extracellular phospholipases of Candida albicans.