慢性肾功能不全通过下调ABCA1表达影响巨噬细胞内胆固醇含量

Chronic renal failure influences macrophages' cholesterol content by down-regulating ABCA1

目的探讨慢性肾功能不全患者巨噬细胞内胆固醇含量的变化及ATP结合盒受体A1(ATP binding cassettetransporter-A1,ABCA1)的表达情况。方法选取2010年8月至2012年3月重庆医科大学第一附属医院住院的慢性肾功能不全(chronic renal failure,CRF)患者30例和健康对照组20例。分离外周血单核细胞诱导贴壁巨噬化,油红O染色鉴定,应用酶法测定巨噬细胞内胆固醇含量,应用real-time PCR与Western blot方法测定细胞ABCA1的mRNA及蛋白表达。结果慢性肾功能不全患者巨噬细胞内胆固醇含量明显高于健康对照组(P<0.01),ABCA1在慢性肾功能不全患者巨噬细胞中的mRNA与蛋白表达显著低于健康对照组(P<0.01)。结论慢性肾功能不全患者巨噬细胞的胆固醇含量明显增加,胆固醇含量增加与慢性肾功能不全患者ABCA1的表达下调有关。

Objective To explore the changes of cholesterol content and the expression of ABCA1 in peripheral macrophages from chronic renal failure （CRF） patients. Methods A total of 30 CRF patients hospitalized in our department from August 2010 to March 2012 and 20 sex- and age-matched healthy volunteers were enrolled in this study. Peripheral blood mononuclear cells （PBMCs） were isolated and stimulated to make them transfer to macrophages. Maerophages were identified by oil red O staining. The cellular cholesterol content was measured by enzymatic colorimetry. Macrophages were collected to detect the expression of ABCA1 by real-time PCR and Western blotting. Results The content of intracellular cholesterol in the peripheral macrophages from CRF patients was significantly higher than that from healthy patients （P 〈 0. 01 ）, while, the expression of ABCA1 was significantly decreased （P 〈 0.01 ）. Conclusion CRF increases cholesterol content in peripheral macrophage, which might be associated with the decreased expression of ABCA1.