黑草药材HPLC指纹图谱的研究

HPLC fingerprint study of the herb of Buchnera cruciata

目的:以不同产地的黑草为研究对象,建立黑草药材的HPLC指纹图谱,为科学评价和有效控制黑草药材的质量提供科学依据。方法:以木犀草素成分为参照峰,采用HPLC法建立黑草药材的指纹图谱;色谱柱:Agilent Extend-C18(250 mm×4.6mm,5μm),流动相:乙腈与0.7%冰醋酸,梯度洗脱;流速:1.0 mL·min-1;检测波长:350 nm;柱温:35℃;进样量:10μL。结果:10批次黑草药材中共有12个指纹图谱峰,经相似度计算,整体相似性好。结论:本文建立的指纹图谱有较强的针对性,为黑草药材的研究以及制定黑草药材的质量控制标准提供科学依据。

Objective： To establish the HPLC fingerprint of the herb of Buchnera cruciata Hamilt. from different regions, and provide the scientific foundation for quality evaluation and quality control of the crude drug. Methods： HPLC method was adopted and luteolin was used as an internal standard compound to build the HPLC fingerprint. The Agilent Extend- C18 column（250 mm × 4. 6 mm,5μm）was used, UV detection wavelength was set at 350 nm, and the column temperature was 35 ℃. The mobile phase was a linear gradient of acetonitrile and 0. 7% glacial acetic acid at the flow rate of 1.0 mL · min-l. Results： Twelve peaks were identified in HPLC fingerprint of ten batches samples of B. cruciata, and the overall similarity was good according to the similarity calculation. Conclu- sion： The fingerprint was specific and the established method can be used for research and quality control of the crude drug of B. cruciata.