出芽短梗霉黑色素的抗氧化活性研究

Study on antioxidant activities of melanin of Aureobasidium pullulans

建立了还原力体系、DPPH自由基体系、羟基自由基体系、超氧阴离子自由基体系、铁离子螯合力体系及抑制脂质过氧化体系。以VC作为参照,研究了出芽短梗霉黑色素在不同体系下的抗氧化活性。结果表明,黑色素具有一定的还原能力,在浓度为0.4g/L时,其还原力与VC相当,黑色素对羟自由基、超氧阴离子自由基、DPPH自由基均有较好的清除作用,同等浓度条件下黑色素清除羟自由基和超氧阴离子的能力均高于VC。此外,黑色素具有一定的铁离子螯合能力和抑制脂质过氧化能力。综上所述,黑色素具有一定的抗氧化活性。

In order to evaluate the antioxidant activities of melanin in Aureobasidium pullulans, reaction systems of reducing force, DPPH free radicals, hydroxyl free radicals, super oxide anion free radicals, iron chela force as well as inhibition of lipid peroxidation were established. Then antioxidant activities of melanin of Aureobasidium pullulans and Vc were measured and compared in different reaction systems. The result indicated that melanin had certain reductive force, in 0.4g/L was equal to the Vc. Melanin could effectively scavenge DPPH free radicals, hydroxyl free radicals and super oxide anion free radicals. Under the condition of the same concentration, melanin remove hydroxyl free radicals and super oxide anion free radicals were higher than Vc. In addition, melanin had certain iron ion chelated force and suppress the lipid peroxidation ability. So, melanin in Aureobasidium puflulans had good antioxidant activities.