超声波辅助酶法提取油茶籽油的影响因素研究

Study on impact factors of the ultrasound-assisted enzymatic aqueous extraction of oil from tea seed

主要研究了超声波技术辅助水酶法提取油茶籽油的工艺。比较不同粉碎条件、不同超声条件及多种酶的油茶籽油提取率,确定最佳工艺:物料粉碎粒度为0.600mm,超声功率400W,超声温度40℃,超声时间40min;最适酶依次为:Alcalase 2.4L>Cellulase>Protex 7L>Viscozyme L。在最佳工艺条件下,游离油提取率达到59.23%,总油提取率达到94.56%。

The present study aimed to evaluate the process of ultrasound-assisted enzymatic aqueous extraction of oil from tea seeds. The optimization procedure was performed on different crushing conditions,ultrasound conditions, and enzymes. The optimized parameters were as follows： crushing particle size 0.600mm, ultrasonic power 400W, ultrasonic temperature 40℃, ultrasonic time 40min. The order of enzyme efficiency was AIcalase 2.4L〉Cellulase〉Protex 7L〉Viscozyme L. Under the above optimum conditions,the free oil extraction rate was 59.23%, and the total oil extraction rate was 94.56%.