泛耐药鲍曼不动杆菌基于管家基因和水平转移基因的菌株亲缘性分析

Phylogenetic analysis based housekeeping genes and horizontal transfer resistance genes in pandrug-resistant Acinetobacter baumannii

目的调查20株泛耐药鲍曼不动杆菌(pandrug-resistant Acinetobacter baumannii,PDR-ABA)的菌株亲缘性。方法收集2010年3月到2010年5月临床标本中分离的PDR-ABA菌20株,采用聚合酶链反应(PCR)的方法检测3种管家基因和37种β-内酰胺类获得性耐药基因、15种氨基糖苷类获得性耐药基因、5种喹诺酮类获得性耐药基因和8种可移动遗传元件等水平转移基因检测,并对检测结果作了样本聚类分析。结果本组PDR-ABA菌carO、gyrA和parC3种管家基因均存在突变,且突变形式一致;blaTEM、blaADC-like、blaOXA-23、aac(6')-Ⅰb、ant(2")-Ⅰ、ant(3")-Ⅰ、aphA1、adeB、tnpU、ISCR1、IS26和ISaba1基因全部阳性,blaCARB-2、blaDHA-1和IS903基因阳性率为5%。样本聚类分析显示本组PDR-ABA菌为克隆传播。结论本组PDR-ABA菌为医院内克隆传播,应加强控制。

Objective To investigate the phylogeny of 20 strains of pandrug-resistant Acinetobacter baumannii （PDR-ABA）. Methods From March 2010 to May 2010, 20 strains of PDR-ABA were collected from clinical specimen. Then, 3 kinds of housekeeping genes, 57 kinds of acquired resistance-related genes to beta-lactams, aminoglycosides, quinolones and 8 kinds of genetic markers of mobile genetic element were analyzed by PCR and the phylogenetic analysis was performed. Results All 20 strains of PDR-ABA were existed mutations for 3 kinds of housekeeping genes and positive for blarEM, b[anoc.like, blaoxn.23, aac（6＇）- I b, ant（2 ＂）- I, ant（3 ＂）- I, aphA1, adeB, tnpU, ISCR1, IS26, 1Sabal, and the positive rate of genes of blaCARB-2, blaDKA.1 and IS903 were 5.0%. Sample cluster analysis showed that this group of PDR-ABA were clonal spread. Conclusions This group of PDR-ABA were clonal spread and the outbreak should be controlled.