人肺癌组织E-cadherin和PTEN及HIF-1α蛋白表达临床意义的探讨

Expression and significance of E-cadherin,PTEN and HIF-1α proteins in human lung cancer tissues

目的:探讨E-cadherin(E-cd)、PTEN和HIF-1α蛋白在肺癌组织中表达及其临床意义。方法:应用免疫组化方法检测99例肺癌组织E-cd、PTEN和HIF-1α蛋白的表达情况。结果:E-cd、PTEN和HIF-1α蛋白在99例肺癌组织中的阳性率分别为63.6%(63/99)、62.6%(62/99)和56.6%(56/99),在癌旁正常组织中的阳性表达率分别为100%(20/20)、100%(20/20)和0,差异均有统计学意义,P<0.05。E-cd蛋白在细支气管肺泡癌组的表达高于小细胞癌组,而HIF-1α蛋白在细支气管肺泡癌组的表达低于小细胞癌组,差异均有统计学意义,P<0.05;其余各组织学类型之间3种蛋白表达差异均无统计学意义,P>0.05。E-cd和PTEN蛋白在淋巴结无转移组的表达均高于淋巴结转移组,P<0.05;而HIF-1α蛋白在两组间的表达差异无统计学意义,P>0.05。E-cd和PTEN蛋白在Ⅰ～Ⅱ期的表达高于Ⅲ～Ⅳ期,P<0.01;而HIF-1α蛋白在Ⅰ～Ⅱ期的表达低于Ⅲ～Ⅳ期,P<0.05。E-cd蛋白表达与PTEN蛋白表达呈正相关,P<0.05;HIF-1α蛋白与E-cd、PTEN蛋白表达均呈负相关,P<0.05。结论:E-cd、PTEN和HIF-1α蛋白的异常表达可能在肺癌的恶性演进中起重要作用,3者在肺癌的侵袭转移中可能起某种协同作用。

OBJECTIVE： To investigate the clinicopathologic significance of the expression of E-cadherin, PTEN and HIF-1α proteins in lung cancer tissues. METHODS： The expression of E-cadherin,PTEN and HIF-1α proteins were detected in 99 cases of lung cancer tissues by immunohistochemical method. RESULTS：The total positive rates of E-cadherin, PTEN and HIF-1α proteins were 63.6% （63/99）, 62.6% （62/99） and 56.6% （56/99）respectively, and 100% （20/20）, 100% （20/20） ,0 in the para-eancino- ma tissues. The significant differences were observed between them （P〈0.05）. In the group of BAC,the positive rate of E-cad herin protein was higher than that in the group of SCLC. While the positive rate of HIF-1α protein was lower than that in the group of SCLC. The significant statistic differences of E cadherin and HIF-1α proteins expression were observed between the groups of BAC and SCLC （P〈0.05）. The expression of the three proteins in other groups had no significant differences （P〉 0.05）. Higher positive rates of E-cadherin and PTEN proteins were obtained in the group of non-lymph-node metastasis than that in group of positive lymph node metastasis. The significant statistic differences were observed between them （P〈0.05）, but no significant differences was found in HIF-1α protein （P〉0.05）. The higher positive rates of E-cadherin and PTEN proteins were obtained in the group of stageⅠ-Ⅱ than that in the group of stage Ⅲ-Ⅳ （P〈0. 01）. Lower positive rate of HIF-1α protein was obtained in the group of stageⅠ-Ⅱ than that in the group of stage Ⅲ-Ⅳ（P〈0.05）. E-cadherin protein expression was positively associated with PTEN expression （P〈0.05）;but HIF-1α protein expression was negatively associated with E-cadherin and PTEN expression （P〈0.05）. CONCLUSIONS： The abnormal expression of E-cadherin, PTEN and HIF-1α proteins may play im portant roles in the malignant progression of lung cancer. The three proteins may play a combined role in invasion and metastasis of lung cancer.