胃癌组织ABCG2和P-gp蛋白表达及其临床意义分析

Expressions and clinical significance of ABCG2 and P-gp protein in gastric carcinoma tissues

目的:探讨中晚期胃癌组织中ABCG2和P-gp蛋白的表达及其临床意义。方法:采用免疫组化法检测60例中晚期胃癌组织和对应的癌旁正常组织中ABCG2和P-gp蛋白的表达情况,分析其与临床病理特征的关系。结果:ABCG2主要定位于细胞膜,胞质中亦有表达,P-gp定位于细胞膜和细胞质,以细胞质为主。ABCG2和P-gp在胃癌组织中的阳性表达率分别为95.0%(57/60)和85.0%(51/60),显著高于癌旁正常组织的81.7%(49/60)和25.0%(15/60),χ2值分别为12.264和52.286,P值分别为0.007和0.000;二者在癌中的共表达率为81.7%(49/60),在表达强度上差异有统计学意义(χ2=28.381,P=0.000),且呈正相关(r=0.156),但差异无统计学意义,P=0.233;在表达程度上一致性差(Kappa=0.084),且差异无统计学意义,P=0.282;其表达均与肿瘤TNM分期、分化状态、浸润深度密切相关(P值均=0.000),与患者年龄、性别、肿瘤大小、有无淋巴结和远处转移无关,P值均>0.05。结论:ABCG2与P-gp作为胃癌检测中的敏感指标,可能成为胃癌诊断、恶性程度、肿瘤进展、耐药、疗效及预后的新指标。

OBJECTIVE：To investigate the expressions and clinical significance of ABCG2 and P-gp protein in the middle-late stage gastric cancer tissues. METHODS：The immunohistochemical method was used to detect the expressions of ABCG2 and P-gp protein in 60 cases of middle-late stage gastric cancer tissues and corresponding adjacent normal tis- sues,and their relationships with clinicopathological features were analyzed. RESULTS： ABCG2 positive stain was mainly located in cytomembrance, and could be in cytoplasmtoo, while P-gp in cytomembrance and cytoplasm, but mainly in the latter. The positive expression rate ofABCG2 and P-gp in gastric cancer tissues was 95.0%（57/60）,85.0%（51/60）,sig- nificantly higher than that in adjacent normal tissues of 81. 7% （49/60）, 25. 0%（15/60,X2 were 12. 264 and 52. 286, P were 0. 007 and 0. 000）;Their co-expression rate in the cancer was 81.7%（49/60） and the difference was statistically significant on the expression degree （ X2 = 28. 381, P = 0. 000 ）, with a positive correlation （ r： 0. 156 ）, but there was no statistical significance（ P： 0. 233）. They had a poor coherence at the expression extent （Kappa--0. 084 ）, with no statisti- cal significance（P：0. 282）. Their expressions were both closely correlated with the tumor TNM stage, differentiation state and invasion depth （all P=0. 000）,but not related to patients＇age,gender,tumor size,with or without lymph node and distant metastasis Call P〉0.05）. CONCLUSION：ABCG2 and P-gp as a sensitive index in the testing of gastric canc- er, may become a new indictor for the diagnosis of gastric cancer,evaluation of malignant degree, tumor progression, drug resistance and efficacy and prognosis.