结直肠癌组织HDAC1 mRNA表达临床意义的探讨

Expression of HDAC1 mRNA in colorectal cancer tissues

目的:探讨HDAC1mRNA在结直肠癌组织中的表达并分析其临床意义。方法:RT-PCR检测50例结直肠癌组织、50例癌旁组织、20例正常结直肠组织和20例腺瘤组织中HDAC1mRNA表达情况,统计学分析HDAC1mRNA相对表达量与患者临床病理特征关系。结果:癌组织HDAC1mRNA相对表达量(0.608±0.032)明显高于癌旁组织(0.496±0.026),腺瘤组织HDAC1mRNA相对表达量(0.495±0.052)明显高于正常组织(0.388±0.050);癌组织中HDAC1mRNA相对表达量与患者的性别(t=0.264,P=0.793)、年龄(t=0.116,P=0.908)和肿瘤大小(t=0.621,P=0.537)无关,而与组织Duke分期(t=3.395,P=0.001)、组织学分型(t=2.070,P=0.043)和淋巴结转移(t=2.162,P=0.036)相关。Duke C、D期HDAC1mRNA相对表达量明显高于Duke A、B期,高、中分化结直肠癌组织HDAC1mRNA相对表达量明显低于低、未分化组织,淋巴结转移患者HDAC1mRNA相对表达量增高。CK20(t=2.389,P=0.021)和Ki-67(t=2.117,P=0.039)高表达患者HDAC1mRNA相对表达量明显增高。结论:HDAC1mRNA高表达与结直肠癌患者临床病理学特征相关。RT-PCR检测HDAC1mRNA在结直肠癌组织中的表达,可能成为一种新的结直肠癌特异性诊断标志。

OBJECTIVE：To study the expression of HDAC1 mRNA in colorectal cancer and analysis its correlation with clinicopathological features. METHODS： The expression of HDAC] mRNA was detected in 50 cases of colorectal cancer and their corresponding adjacent tissues,20 cases of normal tissues and 20 cases of adenoma tissues by R＇F-PCR. The correlation with clinicopathological features was analized by Chi-square test. RESULTS： HDAC1 mRNA expresskm was higher in colorectal cancer（0. 608±0. 032） than that in cancer corresponding adjacent tissues（0. 496±0. 026）,and ad enoma tissues（0.495_＋_0.052） had a higher expression of HDAC1 mRNA than that of normal tissues（0. 388±0. 050）. The expression of HDAC1 mRNA in Duke A and B stages（t=3. 395,P=0. 001） was significantly lower than that in Duke and D stages. The expression of HDAC1 mRNA in lowly- and non-differentiated adenoma（t = 2. 070, P〈 0. 043） was significantly higher than that in the highly and moderately-differentiated adenoma. In addition,the expresskm of HDAC1 mRNA was correlated with lymph node metastasis（t=2. 162,P=0. 036） ,but not with age（t=0.116,P=0. 908） ,sex（t= 0.26,1,P=0.793） and tumor sizes（t=0.621,P--0.537） of patients. The expression of HDAC1 mRNA has a positive correlation with Ki-67（t 2. 117,P=0. 039） and CK20（t=2. 389,P=0.021） expression in cancer tissue. CONCLU- SIONS： HDAC1 mRNA overexpression in colorectal cancer indicates an unfavorable clinicopathological feature. Detection of HDAC1 mRNA may be a new diagnostic method in patients with colorectal cancer .