反复冻融-酶法制备脱细胞膀胱黏膜下基质

Preparation of acellular urinary bladder submucosa by freeze-thawed enzymatic method

目的探讨脱细胞膀胱黏膜下基质的制备方法。方法分别采用反复冻融一酶法和除垢剂一酶法制备脱细胞膀胱黏膜下基质。将原代培养的口腔黏膜细胞接种于两种方法制备的脱细胞膀胱黏膜下基质上，观察细胞生长情况，接种14d后，对基质进行病理切片，皿染色观察。结果口腔黏膜细胞可在反复冻融一酶法制备的脱细胞膀胱黏膜下基质上生长，并形成连续单层细胞。除垢剂一酶法制备的脱细胞膀胱黏膜下基质上无细胞生长。结论反复冻融．酶法可制备适于细胞黏附生长的脱细胞膀胱黏膜下基质。

Objective To investigate a method for the preparation of acellular urinary bladder submuco- sa. Methods Acellular urinary bladder submueosa was derived by two different methods, the freeze thawed enzymatic method and the detergent enzymatic method. Primarily cultured oral keratinocytes were inoculated on the two kinds of perpared acellullar urinary bladder submueosa. Specimens were harvested at 14 days for histo- logical observation by HE staining. Results Continous single layer oral epithelium was formed on the surface of matrix derived by freeze-thawed enzymatic method. The oral keratinocytes could not adhere and proliferate on the matrix derived by detergent enzymaticmethod. Conclusion Aeellular urinary bladder submucosa suitable for cell adhesion and proliferation could be derived by freeze thawed enzymatic method.