高效液相色谱法评价大孔树脂纯化甘草酸工艺

Purification of Glycyrrhizic Acid from Glycyrrhiza Using Macroporous Resin

对甘草纯化甘草酸的工艺进行系统研究。通过对DA201-C和AB-8两种型号大孔树脂静态吸附和解吸的比较,选择AB-8型大孔树脂对甘草酸进行纯化;静态吸附解吸实验表明,AB-8型大孔树脂纯化甘草酸的最佳工艺条件为上样液pH6.0、上样液质量浓度5.89mg/mL、洗脱液50%乙醇溶液;应用高效液相色谱法检测甘草酸纯度的方法,结果显示,经AB-8型大孔树脂纯化后的甘草酸纯度52.3%,重结晶后纯度为76.0%。

The purification of glycyrrhizic acid from glycyrrhiza using macroporous resin was study. Static absorption and desorption experiments were carded out to screen the optimal resin from macroporous resins DA201-C and AB-8. AB-8 was found to have better performance than DA201-C. In static adsorption and desorption experiments, the optimal purification conditions of glycyrrhizic acid by resin AB-8 were sample pH 6.0, 5.89 mg/mL sample concentration, and 50% ethanol as the desorption solvent. In addition, the purity of purified glycyrrhizic acid was determined by HPLC to be 52.3%, and was increased to 76.0% after recrystallization.