摘要
建立食品中36种合成色素同时测定方法。样品经热水提取,采用聚酰胺粉净化,超高效液相色谱用甲醇-5mmol/L乙酸铵溶液梯度淋洗,1.8μm C18短柱分离,在电喷雾离子源负离子模式下采用飞行时间质谱检测。36种合成色素线性范围在0.01～1μg/mL,检测低限在0.0072～0.94mg/kg之间,加标回收率在80%～106%范围,标准偏差在1.8%～9.7%之间。
An ultra high performance liquid chromatography and time-of-flight mass spectrometry （UPLC-TOF-MS） method for the determination of 36 artificial pigments in foods was established. Samples were extracted with hot water and purified with polyamide powder, and the extract was separated on C18 column （1.8 μm） using methanol-5 mmol/L ammonium acetate as the mobile phrase through gradient elution and analyzed by UPLC-TOF-MS coupled with electrospray ionization （ESI） resource in a negative ion mode. An excellent linear relationship for pigments was observed in the concentration range of 0.01-1 μg/mL and the detection limit was 0.0072--0.94 mg/kg. The recovery rate of spiked samples was in the range of 80%-106% with relative standard deviation of 1.8%--9.7%.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第6期215-218,共4页
Food Science
基金
福建省自然科学基金重点项目(2010Y0001)
福建检验检疫局科技项目(FK2008-21)
关键词
飞行时间质谱
超高效液相色谱
合成色素
食品
高通量
time-of-flight mass spectrometry （TOF-MS）
ultra pressure liquid chromatography （UPLC）： artificial pigment
food
high throughout
