心外膜脂肪组织中他扎罗汀诱导基因2表达与冠状动脉粥样硬化相关性的研究

Relation between chemerin expression in epicardial adipose tissue and coronary atherosclerosis

目的探讨他扎罗汀诱导基因2(chemerin)在冠心病(CHD)患者心外膜脂肪组织(EAT)中表达及与冠状动脉粥样硬化(CAS)的关系。方法选取53例行选择性心脏外科手术的患者,根据冠状动脉造影结果分为冠心病组(CHD,n=37)和非冠心病组(NCHD,n=16),收集临床资料,进行Gensini冠状动脉评分。体外循环前分别取两组EAT和皮下脂肪组织(SAT),采用RT-PCR法检测脂肪组织中chemerin、ChemR23、APN与TNF-αmRNA的表达。结果与NCHD组比,CHD组EAT中chemer-in、TNF-α表达水平升高,APN表达减低(P均<0.01),但两组间ChemR23表达差异无统计学意义(P>0.05);CHD组chemerin在EAT中表达高于SAT(P<0.05),NCHD组chemerin表达差异无统计学意义(P>0.05);EAT中chemerin基因表达与Gensini冠状动脉评分、BMI、WC、FPG呈正相关,与APN表达呈负相关。结论 CHD患者EAT中chemerin表达升高,CAS越严重,chemerin基因表达水平越高。

Objective To explore the correlation between the chemerin expression in epicardial adipose tissue （EAT） and coronary heart disease （CHD）. Methods Fifty-three patients undergoing selective heart surgery were chosen and divided into CHD group （n= 37） and non-CHD group （n = 16） according to the result of coronary angiography, then the clinical data were collected and the coronary artery Gensini scoring was carried out. The EAT and thoracic subcutaneous adipose tissue （SAT） were obtained before eardiopulmonary bypass. And the expressions of chemerin, chemR23, APN, and TNF-α in adipose tissue were detected by RT-PCR. Results Compared with the non-CHD group, the expressions of chemerin （P〈0. 01） and TNF-α （P〈0. 01） of the CHD group were significantly higher, the adiponectin expression was significantly lower （P〈0.01）, but there was no difference in the chemR23 expression in EAT between two groups （P〈0. 05）. In the CHD group, the chemerin expression in EAT was significantly higher than in SAT （P〈0.05）, however, such difference did not exist in the non-CHD group （P〈0.05）. The chemerin gene expression in EAT was positively correlated with Gensini score （r= 0. 365, P〈0. 05）, BMI （r=0. 305, P〈0.05）, waist circumference （r=0. 384, P〈0.01）, and fasting blood glucose （r =0. 334, P 〈 0. 05）, while negatively correlated with adiponectin expression （r =-0. 322, P〈0. 05）. Conclusion Chemerin expression in EAT of patients with CHD is significantly elevated, and the more severe the coronary atherosclerosis is, the higher the chemerin gene expression is.