普罗布考和瑞舒伐他汀对阿霉素诱导心力衰竭大鼠心肌细胞外整合素β1和β3作用的实验研究

Effect of Probucol and Rosuvastatain on Myocardial Extracellular Integrin β1 and Integrin β3 in Adriamycin-induced Heart Failure Rats

目的:研究普罗布考(Probucol)和瑞舒伐他汀(Rosuvastatain)对阿霉素诱导心力衰竭大鼠心肌细胞外整合素β1(Integrinβ1)和整合素β3(Integrinβ3)的作用。方法:雄性Wistar大鼠120只,重250±50 g,随机选取15只入空白对照组(Normal组),余105只给予阿霉素诱导心力衰竭。首次给药2个月后存活心衰大鼠随机分成3组:瑞舒伐他汀组(ROS组)、普罗布考组(PRO组)、心衰组(ADR组),将ROS组、PRO组大鼠分别以瑞舒伐他汀,普罗布考进行药物干预,Normal组、ADR组给予蒸馏水灌胃,连续干预4周后处死取材,心脏标本经HE染色、电镜观察大体及超微结构、Masson染色后计算胶原容积分数;免疫组织化学标记左室心肌细胞外整合素β1和β3,应用计算机病理图像分析系统的组化分析模块进行图像分析,计算Integrinβ1及Integrinβ3的相对含量。结果:与ADR组相比,PRO和ROS组大鼠左室胶原容积分数(CVF)显著降低(P<0.05),同时Integrinβ1及Integrinβ3的相对含量显著降低(P<0.05)。结论:PRO及ROS能够显著降低心肌细胞外基质(ECM)Integrinβ1及Integrinβ3的含量,从而明显改善心力衰竭大鼠的左心室功能。

Objective： To investigate the effect of probucol and rosuvastatain on myocardial extraceUular integrin β1 and β3 in adriamycin-induced heart failure rats. Methods： 120 male wistar rats, weight 250±50 g, fifteen rats were chosen as normal group, the others were induced heart failure by adriamycin. After two months, the living rats were randomly divided into three groups： adriamycin group, rosuvastatain groug, probucol group. All rats were killed after 4 weeks intervention. Morphological characteristics were measured with HE, Masson and electron microscope. Collagen volume fraction （CVF） was assessed with computer-assisted image analysis system. Integrin β1 and integrin β3 were assessed by immunohistochemistry staining. Results： CVF of PRO and ROS groups decreased obviously compared with that in the ADR group. Both integfin β1 and β3 decreased in PRO and ROS groups （P 〈0.05）. Conclusions： PRO and ROS can reduce integrin β1 and β3 so that they can improve the left ventricular function of heart failure wistar rats which are induced by adriamycin.