ABCA1和PPARγ与糖尿病动脉粥样硬化关系的研究

Study on the Relationship between ABCA1 and PPARγ in Diabetic Atherosclerosis

目的:研究ATP结合盒转运体A1(ABCA1)在多种糖尿病特有因素刺激下在巨噬细胞中的表达,以及PPARγ激动剂干预后其表达的变化,探讨ABCA1及PPARγ在糖尿病大血管并发症发展中的作用机制。从而为研究糖尿病大血管并发症的发生机制及防治提供一定的理论依据。方法:以巨噬细胞为研究对象,体外模拟糖尿病状态,分别以高葡萄糖、高胰岛素和糖基化终末产物刺激巨噬细胞,检测细胞中ABCA1表达的变化;以PPARγ激动剂预处理巨噬细胞后,再以上述因素刺激细胞,分别检测巨噬细胞中ABCA1的表达并比较。结果:高葡萄糖、高胰岛素和糖基化终末产物(AGE)可作为独立因素,导致细胞中ABCA1表达减少(P<0.05)。PPARγ激动剂预处理后,ABCA1表达量增加(P<0.05)。结论:糖尿病状态下,一些糖尿病特有的刺激因素如:高葡萄糖、高胰岛素和糖基化终末产物等作为独立因素使ABCA1表达减少,可能是糖尿病患者动脉粥样硬化发生率较非糖尿病人群增高的原因。PPARγ激动剂干预后,糖尿病状态下ABCA1的表达增加,这提示我们应用PPARγ激动剂可能延缓糖尿病患者动脉硬化进展。

Objective： To investigate the effects of various diabetic stimulating factors on the expression of ATP-binding cassette A1 （ABCA1） in macrophages. The effect of PPARγ/ receptor agonist on expression of ABCA1 in macrophages.In order to inquire the mechanisn of ABCA1 and PPARγ on diabetic atherosclerosis. Methods： Macrophages was incubated with high glucose, high insulin and advanced glycosylation end products （AGE）; simultaneously, the cells pre-treated with PPARγ receptor agonist was incubated with above factors, the expression of ABCA1 was detected and compared. Results： Three factors inhibited the expression of ABCA1 independently （P〈0.05）; PPARγ/receptor agonist increased the expression of ABCAI induced by three factors istinctly （P〈0.05）. Conclusions： The expression of ABCA1 can be inhibited by these stimulating factors on diabetic state. The reason may be that more people on diabetes have atherosclerosis than normal.PPAR-y receptor agonist promotes the expression of ABCA1 in macrophages in diabetes, and can delay the process o f diabetic atherosclerosis.