姜黄素对大鼠子宫内膜异位症影响的实验研究

Effect of crucumin on expressions in endometriosis rats

目的探讨姜黄素治疗大鼠子宫内膜异位症的治疗机制。方法采用清洁级雌性SD大鼠子宫内膜自体移植法建模,3周后,将建模成功的大鼠随机分为模型组(n=10)、孕三烯酮组(n=9)、姜黄素低剂量组(n=9)、姜黄素中剂量组(n=8)、姜黄素高剂量组(n=9);将行假手术大鼠做空白对照组(n=10),给药3周,ELISA法检测血清雌二醇(E2)的表达,SP免疫组织化学法检测异位子宫内膜组织雌激素受体(ERα)、细胞增殖核抗原(PCNA)和血管内皮生长因子(VEGF)的表达。结果模型组血清雌二醇和异位组织ERα、PCNA、VEGF表达比空白对照组明显增高(P<0.01);治疗组表达量较模型组明显降低(P<0.05);结论姜黄素能降低大鼠血清雌二醇的表达水平的同时降低子异位内膜组织ERα、PCNA,VEGF表达达到抑制异位内膜的生长与血管生成而实现治疗目的。

AIM To investigate the effect of crucumin on rats with endometriosis.METHODS The endometriosis model was induced in clean female SD rats by surgery of self-endometrium transplant.After three weeks,the model rats were divided into four groups that were model group（n=10）,gestrinone group（n=9）,low dose group of crucumin（n=9）,middle dose group of crucumin（n=8）,high dose group of crucumin（n=9）,and sham operation as control group（n=10）.After three weeks of treatment,the expression of serum estradiol was measured by ELISA,and the expressions of estrogen receptor-α（ERα）,proliferating cell nuclear antigen（PCNA） and vascular endothelial growth factor（VEGF） were measured by immunostaining.RESULTS As compared with the control group,the level of estradiol in serum and levels of ERα,PCNA and VEGF in ectopic tissues in model group increased（P＆lt;0.01）,but those in gestrinone and crucumin groups decreased（P＆lt;0.05）.CONCLUSION Curcumin can reduce level of serum estradiol expression and the ERα,PCNA,VEGF expression through inhibiting the angiogenic potential of endometriosis in rats.