HPLC法同时测定决明子中6种蒽醌类成分

Determination of six anthraquinones in Cassiae Semen by HPLC

目的建立决明子中橙黄决明素、芦荟大黄素、大黄酚、大黄素、大黄酸、大黄素甲醚6个成分的HPLC测定方法,比较生、炒决明子中化学成分含有量的差别。方法采用Kromasil C18色谱柱(150 mm×4.6 mm,5μm),以乙腈(A)-0.1%磷酸水溶液(B)为流动相,梯度洗脱,体积流量为1.0 mL/min检测波长为284 nm。结果生、炒决明子中6个成分橙黄决明素、芦荟大黄素、大黄酚、大黄素、大黄酸、大黄素甲醚进样量分别在0.114 4～1.144μg(r=0.999 2),0.037 60～0.376 0μg(r=0.999 1),0.166 0～1.660μg(r=0.999 0),0.024 2～0.242 0μg(r=0.999 4),0.252 8～2.528μg(r=0.999 4),0.035 20～0.352 0μg(r=0.999 2)范围内与峰面积呈良好线性关系;平均回收率(n=5)分别为97.0%、98.4%、98.0%、98.2%、97.1%、97.8%。结论决明子经炒制后6个蒽醌类成分的量降低。

AIM To set up a method for the determination of aurantio-obtusifolin,aioe-emodin,rhein,emodin,chrysophanol and physcion in Cassiae Semen by HPLC,and to make a comparison between the crude and stirfrying Cassiae Semen from different producing districts.METHODS Chromatographic conditions concluded Kromasil C18（150 mm × 4.6 mm,5 μm） column with the mobile phase consisting of acetonitrile-0.1% phosphoric acid in gradient elution mode at the flow rate of 1.0 mL / min.The detection wavelength was set at 284 nm.RESULTS Aurantio-obtusifolin,aioe-emodin,rhein,emodin,chrysophanol,physcion in Cassiae Semen had good linearity in the ranges of 0.114 4-1.144 μg（r = 0.999 2）,0.037 60-0.376 0 μg（r = 0.999 1）,0.166 0-1.660 μg（r =0.999 0）,0.024 2-0.242 0 μg（r =0.999 4）,0.252 8-2.528 μg（r =0.999 4）,0.035 20-0.352 0 μg（r =0.999 2）,respectively.The average recoveries of six constituents were 97.0%,98.4%,98.0%,98.2%,97.1% and 97.8%,respectively.CONCLUSION The contents of six anthraquinones in processed Cassiae Semen decreased.