黄灯笼辣椒种质资源遗传多样性ISSR分析

ISSR Analysis on Genetic Diversity of Capsicum chinense Jacquin Germplasm Resource

采用ISSR分子标记技术对22份国内外黄灯笼辣椒种质的遗传多样性进行了分析,为选配黄灯笼辣椒杂交育种亲本选择提供了参考。根据加拿大哥伦比亚大学公布的100对ISSR引物,从中筛选出17条多态性好、条带稳定的引物,用其对22份黄灯笼辣椒种质进行扩增,共获得154条谱带,其中140条为多态性条带,多态性比率为90.9%,表明ISSR标记对黄灯笼辣椒具有较高的多态性;通过对黄灯笼辣椒ISSR遗传相似系数的统计与分析,品种间的遗传相似系数在0.461～0.870之间,平均为0.778。聚类分析表明,22份材料在遗传相似系数0.52处分为2类,其中L508为种间杂种后代,与其它种质具有较远的亲缘关系,单独为一类;在遗传相似系数0.72处可以分为4类,把不同亲缘关系的种质明显区分开来,表明ISSR分子标记进行黄灯笼辣椒遗传多样性的分析是可行的。黄灯笼辣椒品种间具有较丰富的遗传多样性,并为黄灯笼辣椒品种的选育和种质资源的保护提供了理论依据。

In this study, the genetic diversity of 22 capsicum were analyzed to screen parents for hybrid breeding. According to the 100 pairs of ISSR primers from University of British Columbia Biotechnology, the 17 effective primer pairs were selected to amplify 22 Capsicum cuhivars. There were 154 fragments obtained by amplification of ISSR, and 140 fragments were polymorphic. The results indicated that ISSR molecular analysis had comparatively high polymorphism in Capsicum chinense Jacquin, and the rate of polymorphic reached 90.9%. The coefficient of genetic similarity in different cuhivars was ranged from 0.461 to 0.870 with an average of 0.778. The cluster results showed that 22 materials were classified into 2 groups using the genetic similarity coefficient 0.52. L508 was a separate category, a distant relative to other species. And the 22 materials were classified into 4 groups using the genetic similarity coefficient 0.72. The results indicated that ISSR markers was an available method to study the genetic diversity of Capsicum chinense Jacquin germplasm resources. The 22 Capsicum chinense Jacquin cultivars possess abundant genetic diversity of populations.