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海南番木瓜花叶病毒全长cDNA克隆及序列分析 被引量：1

Complete Genomic Sequence of Papaya mosaic virus Isolate from Hainan Island,China
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摘要 提取海南果园番木瓜病叶样品总RNA,反转录得到cDNA第一链,基于已报道的番木瓜花叶病毒(Papaya mosaic virus,PaMV)全长基因组序列,设计引物,PCR扩增PaMV-CP基因序列,通过测序和序列比对分析确定发病植物感染了PaMV。再通过RT-PCR扩增PaMV全长cDNA序列,克隆及测序结果表明,所获得的cDNA全长为6 656 bp,与国外报道的PaMV分离物全长核苷酸序列的相似性达99.6%。 The complete genomic sequence of Papaya mosaic virus of Hainan isolate （PaMV-HN, GenBank： JX524226）, a member of the genus Potexvirus, was determined, which shared with a 99.6% nucleotide sequence identity with the PaMV isolate from USA （GenBank： NC_ 001748）. The complete genomic sequence of PaMV-HN was 6 656 nucleotides long and the genome contained five typically conserved open reading frames found in members of the genus Potexvirus.
作者 王永辰 沈文涛 王树昌 庹德财 言普 黎小瑛 周鹏
机构地区 海南大学农学院 中国热带农业科学院热带生物技术研究所
出处 《热带作物学报》 CSCD 北大核心 2013年第2期297-300,共4页 Chinese Journal of Tropical Crops
基金 国家自然科学基金项目(No.30560082 31000844) 海南省重点科技计划(No.ZDXM20120027)
关键词 番木瓜花叶病毒 全长CDNA 克隆 序列分析 PaMV Full-length cDNA Cloning Sequece analysis
分类号 S432.41 [农业科学—植物病理学]
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参考文献16

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共引文献3

同被引文献15

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热带作物学报
2013年 第2期

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