香蕉炭疽病优势拮抗酵母菌的分离及筛选

Isolation and Screening of Dominant Antagonistic Yeasts of Banana Anthracnose

采用稀释平板法和平板划线法从8个不同品种的香蕉果实上分离纯化酵母菌,并将其与香蕉炭疽病原菌进行平板对峙培养及离体防效试验,以筛选优势拮抗酵母菌。结果表明:从香蕉果实上共分离纯化酵母菌329株;平板对峙培养后获得3株拮抗香蕉炭疽病原菌作用明显的酵母菌;离体防效试验筛选出1株拮抗效果较好且稳定的优势拮抗酵母菌Z-BR-16,以无菌水为对照,接种7 d后,其1.0×108CFU/mL菌液对香蕉炭疽病的抑制率为75.94%。

Yeast strains were separated and purified from 8 banana species through pour plate and streak plate methods, and dominant antagonistic yeasts were obtained by culturing them with the banana anthrax pathogen through confront culture method and in-vitro test. The result showed that there were 329 yeast strains purified from the banana fruits. The 3 yeast strains which had obvious antagonism to the banana anthrax pathogen were screened out. Finally, The yeast strain Z-BR-16 wich had the best and the most stable antagonistic effect was screened out. Compared with sterile water, the inhibition rate of the yeast strain Z-BR-16 in 1.0×10^8 CFU/ml to the banana anthracnose was up to 75.94% after 7 davs after vaccination.