当归化学成分的分离与鉴定

Isolation and identification of the chemical constituents from roots of Angelica sinensis(Oliv.)Diels

目的研究当归Angelica sinensis(Oliv.)Diels的化学成分,为其药理作用的研究奠定物质基础。方法采用硅胶柱色谱及制备薄层色谱等方法进行分离纯化,根据理化性质和波谱数据(1H-NMR、13C-NMR等)鉴定化合物的结构。结果分离得到13个化合物,分别鉴定为:正丁基苯酞(3-butylphthalide,1)、4羟基3丁基苯酞(4-hydroxy-3-butylphthalide,2)、2-methoxy-2-(4'-hydroxyphenyl)ethanol(3)、反式阿魏酸(ferulic acid,4)、厚朴酚(magnolol,5)、黄芩苷(baicalin,6)、2″O(2甲基丁酰基)异当药黄素[2″O(2-methylbutyryl)-isoswertisin,7]、harman(8)、1-ace-tyl-β-carboline(9)、酒渣碱(flazine,10)、菠菜甾醇(α-spinasterol,11)、异欧前胡素(isoimperatorin,12)、发卡二醇[3(R),8(S)-falcarindiol,13]。结论化合物3、7～9、11为首次从该属植物中分离得到,化合物5为首次从该植物中分离得到。

Objective To isolate and identify the chemical constituents from roots of Angelica sinensis （Oliv. ） Diels. Methods Compounds were isolated by various kinds of column chromatography methods and preparative TLC, and their structures were identified by the physico-chemical characters and the spectral analysis. Results Thirteen compounds were isolated and their structures were identified as：3-butylphthalide （ 1 ） ,4-hydroxy-3-butylphthalide （ 2 ）, 2-methoxy-2 - （4-hydroxyphenyl） ethanol （3）, ferulic acid （ 4 ）, magnolol （ 5 ） , baicalin （ 6 ） , 2- O- （2-methylbutyryl） -isoswertisin （ 7 ） , harman （ 8 ）, 1 - acetyl-β-carboline （ 9 ）, flazine （ 10 ）, a-spinasterol （ 11 ）, isoimperaterin （ 12 ） and 3 （ R）, 8 （S） -falcarindiol （ 13 ）. Conclusions Compounds 3, 7- 8,11 are isolated from Angelica genus for the first time, and compound 5 is isolated from this plant for the first time.